Characterising The Genetic Basis Of Escherichia Coli And Staphylococcus Aureus Interaction By Bivariate GWAS And System Mapping

As an important part of biological system,the interspecific interaction of microorganisms is one of the key factors to determine whether microorganisms can adapt to the change of external environment In the process of interaction,genes play an important role in the formation of community structure Most previous studies have focused on community changes and individual physiological responses Bivariate genome-wide association analysis(GWAS)can obtain significant genes in this study.System mapping can describe the direct,indirect and epistatic effects of bacterial interaction.In this study,Escherichia coli and Staphylococcus aureus,which are related to human health and widely existed in nature,were selected for mono-culture and co-culture.Quantitative real-time PCR was used to obtain phenotypic data of bacteria,High density genetic markers were obtained by resequencing in the whole bacterial genome.Bivariate GWAS were used to obtained the Significant gene,597 and 253 significant genes were found from E.coli and S.aureus,respectively.Some of these genes are found to be involved in biological processes such as cell division,biofilm synthesis,transcription and metabolism.Fifty-four significant pairs of genes were obtained by systematic mapping,It is found that some of the genes are involved in nutritional metabolism,signal transmission,cell division,outer membrane synthesis and other physiological processes of bacteria.Then the direct,indirect and epistatic effects of hub genes yjjW and ggt were analysedIn this study,Bivariate GWAS was used to fit phenotypic data,Compared with traditional GWAS,it has higher accuracy and statistical efficiency,Using this method,we obtained a large number of genes that related to bacterial interactions,This phenomenon suggests that bacterial interactions are not only controlled by a single gene.The method of system mapping found 54 pairs of significant genes in the process of bacterial interaction,proving that different genes in the process of bacterial interaction influenced each other through different genetic effects and jointly contributed to the development of the interaction process.By using two different methods for genome-wide association analysis,this paper explores the genetic mechanism of the interaction between the two bacteria from different perspectives,and explains that the interaction process of bacteria is the result of the interaction of different genes,which provides a theoretical basis for a more systematic and comprehensive understanding of the nature of the interaction between bacteria.