| Developing desalination technology is an effective way to solve the shortage of freshwater resources in the world.With the wide spread,application and innovation of seawater desalination technology in the world,seawater desalination projects also produce a large amount of concentrated wastewater(concentrated brine).If the treatment and utilization of concentrated brine is improper,it will cause immeasurable potential harm to the environment.In this study,the reverse osmosis seawater desalination technology with the highest market share in the world is selected.The concentrated brine of the Lushan reverse osmosis desalination plant in Zhoushan City,Zhejiang Province is selected as the research object,and the salty nature of Dunaliella salina and Artemia is explored.The feasibility of brine culture as a culture medium are for the culture of Artemia,and the effect of different nitrogen and phosphorus concentrations on Dunaliella salina.In this study,the changes of body length and ecological changes of Artemia and the changes of chlorophyll,total sugar,total lipid and ?-carotene in Dunaliella salina were used as indicators.The experimental results of this study are as follows:(1)When concentrated brine is used as the medium for the microalgae,the pretreatment of the conventional water treatment is not required.Dunaliella salina,Chlorella and Artemia can grow normally in concentrated saline environments.Phosphorus concentration had an effect on the growth of Dunaliella salina,total chlorophyll,total sugar and total lipids;Nitrogen concentration had an effect on the growth of Dunaliella salina,total chlorophyll,total lipids and ?-carotene.(2)When the nitrogen concentration was 3.52 mmol/L and the phosphorus concentration was 144 ?mol/L,Dunaliella salina had the best growth and the highest total lipid accumulation.When the nitrogen concentration was 1.76 mmol/L and the phosphorus concentration was 36 ?mol/L,the total chlorophyll accumulation of Dunaliella salina was the highest.When the nitrogen concentration was 7.04 mmol/L and the phosphorus concentration was 36 ?mol/L,the total sugar accumulation of Dunaliella salina was the highest.When the nitrogen concentration was 7.04 mmol/L and the phosphorus concentration was 288 ?mol/L,the ?-carotene accumulation of Dunaliella salina was the most.(3)The effects of three kinds of bait,Chlorella,yeast powder and spirulina powder feeding on the Artemia were not ideal.The effect of Dunaliella salina on feeding Artemia was the most ideal.Chlorella,yeast powder and spirulina powder are fed to Artemia.When yeast powder is fed,the growth rate of Artemia is the largest,followed by spirulina powder and Chlorella.The yeast powder is fed to Artemia.When the mortality rate of Artemia was the smallest,the spirulina powder was the second,and the Chlorella was the smallest.When the spirulina powder was fed to the Artemia,the Artemia still survived at 28 days.(4)Under the condition of Dunaliella salina as the food source,the growth of Artemia has been carried out for three consecutive generations and multiple reproductions of the same individuals,and two offspring of different reproductive methods have been obtained: larvae and dormant eggs;Artemia hatching has been observed.The whole process of ecology such as larvae,adults,oviparous,oviparous,and regrowth.In this study,we explored the use of concentrated brine,which is a huge amount of by-products produced by desalination plants,as a carrier to explore the feasibility of incubation for Artemia eggs and the breeding of Artemia,optimize conditions,solve problems found during the research process,and finally form a complete process system.The research results will provide new ideas and methods for the disposal and utilization of concentrated brine in desalination projects,and its application will create additional economic value for the industry. |
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