Detection And Drug Resistance Analysis Of Escherichia Coli Virulence Genes In Deer Feces Of Diarrhea In Xinjiang Second Division

If diarrhea caused by pathogenic E.coli is not treated in time or the medication is unreasonable,as the course of the disease develops,a stag will form,and in serious cases,the deer will die.Escherichia coli spreads rapidly,and once it becomes ill,it spreads quickly,causing serious economic losses.At present,veterinary clinics mainly use antibiotics to prevent and treat E.coli disease.Because antibiotics are not used for a long time,bacteria have developed drug-resistant bacteria under the pressure of drugs.Due to the different antibiotics used,there are obvious differences in bacterial resistance in different regions and different deer farms.Therefore,the isolation and identification of pathogens and in vitro drug sensitivity tests before treatment can have very important clinical significance in drug selection and prevention,and control of E.coli disease in fawn diarrhea.This experiment mainly collected the anal swab of a deer diarrhea deer with a large scale in the second division of the Xinjiang Production and Construction Corps.The diarrhea samples were isolated and identified for pathogens,pathogenic E.coli specific virulence genes,and mouse Disease tests,in vitro drug sensitivity tests and drug resistance gene detection.Isolation and identification of E.coli.Anal swabs collected from the deer farm of the Second Division of the Xinjiang Production and Construction Corps were isolated and purified using a bacterial selection medium,using micro-biochemical tube detection,pathogenicity test in mice,and pathogenic E.coli Identification methods such as PCR detection of force genes.62 strains of E.coli carrying virulence genes were detected in 105 swallow deer diarrhea anal swabs.Detection of virulence genes.A total of 105 fecal samples of deer's diarrhea were collected from the deer farm of the second division of the Xinjiang Production andConstruction Corps,and a total of 65 strains of E.coli were isolated.h1yA),toxins(afaD-8,estA,estB,Stx1,Stx2,LT-I,LT-II,EAST1),adhesins(K88,K99,987 p,F1,F41,F17,F18,saa,CS31 A,afaE-8)encoding genes and enterohemorrhagic E.coli 0157,H7 antigen genes,a total of 26 pathogenic E.coli-specific virulence genes were tested.Results A total of 62 strains of E.coli carrying virulence genes were detected.Among the 62 isolates isolated,96.8%(60isolates)carried at least one adhesin gene,of which the F1(60/62)gene had the highest detection rate,followed by F17(13/62),CS31A(4/62),afaE-8(2/62),K88(1/62).No K99,F41,F18,987 P and saa genes were detected;62 isolates carry at least one toxin,of which ETT2(59/62)has the highest detection rate,followed by irp2(12/62),eaeA(7/62),LT-II(5/62),hlyA(5/62),ler(4/62);11 strains of H7 antigen were detected.LT-1,O157,Stx1,Stx2,EAST1,aFaD8 and Sep were not detected.Drug sensitivity test.Using K-B paper method,19 kinds of antibiotics commonly used in veterinary clinic were selected to conduct in vitro drug sensitivity test on 62 strains of E.coli isolates.The results showed that the isolates had the highest resistance to clindamycin,medicin and gentamicin,with resistance rates of 85.5%,80.6% and 79.0%,respectively;the isolates were very resistant to florfenicol and cefoperazone Sensitive,the sensitivity rate is100%.PCR amplification of some drug resistance genes.By PCR method,some drug resistance genes that have been researched and reported in quinolones,chloramphenicol,sulfonamides,aminoglycosides,tetracyclines and ?-lactams have been detected.Quinolone detected gyrA,gyrB and parC genes,the positive detection rates were 100%,25.8% and95.2% respectively;the representative genes of chloramphenicol were mainly floR gene and clma gene,and the positive result of floR gene was 8.1%.The positive result of clma gene was 3.2%;the representative gene of sulfonamides was mainly Sul2 gene,and the positive result of Sul2 gene was 29.2%;the representative genes of aminoglycosides were mainly Aph(3')and aadA genes,and the positive results were 14.5% And 12.9%;the representative genes of tetracyclines are mainly tet(B)genes,and the positive result is 6.5%;the representative genes of ?-lactams are mainly blaTEM and blaCTX genes,and the positive results are 30.6%and 29.0%,respectively.Using simple and easy physical and chemical methods,drug sensitivity tests and molecular biological methods to isolate,identify and detect drug resistance genes in E.coli caused by deer diarrhoea in the deer farm of the Second Division of the Xinjiang Production and Construction Corps to initially grasp the large intestine in the area The prevalence of Bacillus and drug resistance provide a scientific test basis for the rapid clinical diagnosis and treatment and reasonable medication of diarrhea Escherichia coli disease.