| Background and purpose:Gliomas are the most common primary malignant tumors of the central nervous system in adults.The World Health Organization?WHO?divides gliomas into I-II low-grade gliomas and III-IV high-grade gliomas.High-grade gliomas account for 75%of adult primary brain tumors.The most aggressive type of glioma,grade IV glioma,also known as glioblastoma,has a 5-year survival rate of only 10%.The standard treatment of glioblastoma is mainly surgical resection to relieve symptoms caused by intracranial hypertension and compression,and postoperative radiotherapy combined with temozolomide adjuvant chemotherapy.However,the efficacy is limited,and its mortality rate has always been the highest in central nervous system malignancies,with a median survival time of about 15 months.Adipocyte enhancer-binding protein 1?AEBP1?was originally thought to be a transcriptional inhibitor that negatively regulates adipogenesis.Subsequently,more and more studies show that AEBP1 plays an important role in the occurrence and development of multiple tumors,and AEBP1 may be considered a proto-oncogene.However,there are few studies on the role of AEBP1in glioblastoma at home and abroad.This study intends to explore the expression of AEBP1 in glioblastoma and its prognosis through comprehensive mining of various databases,and to predict the possible functions and signaling pathways of AEBP1 in glioblastoma.It is hoped to further clarify the possible mechanism of the occurrence and development of glioblastoma from a new perspective,and provide a basis for seeking potential effective targets for the treatment of glioblastoma and prolonging the survival of patients.Methods:The expression of AEBP1 mRNA in GBM was analyzed by Oncomine database and GEPIA website;The GEPIA website and TCGA data were used to analyze the correlation between the level of AEBP1 mRNA and various clinicopathological features,as well as its influence on the prognosis of GBM.Subsequently,the Human Protein Atlas?HPA?database was used to further analyze the expression of AEBP1 protein in gliomas;the co-expressed genes of AEBP1 were further found in the cBioPortal database.The Web Gestalt online tool was used to perform gene ontology?GO?and Kyoto gene and genome encyclopedia?KEGG?pathway enrichment analysis on AEBP1and its co-expressed genes.Results:The 7 data sets screened by the Oncomine database and the analysis of the GEPIA website showed that the expression of AEBP1 mRNA in glioblastoma tissue was up-regulated compared with normal brain tissue,and the difference was statistically significant?P<0.01?;GEPIA website survival analysis showed that patients with high expression of AEBP1 mRNA had lower overall survival?OS?than patients with lower expression,and the difference was statistically significant(P=3.9×10-5).Further TCGA database analysis showed that the expression level of AEBP1 mRNA was related to the mutation of isocitrate dehydrogenase 1?IDH 1??P=0.005?and was an independent influencing factor of OS in patients with glioblastoma(,0.3902-0.8127,P=2.16×10-3);HPA database also confirmed the positive expression of AEBP1 protein in glioma tissues;Finally,KEGG pathway enrichment analysis showed that the major enrichment pathways of AEBP1 and its co-expressed genes include Hippo,NF-?B,TNF and other signaling pathways.Conclusion:High expression of AEBP1 in glioblastoma,and high expression of AEBP1 mRNA is an independent prognostic factor for patients with glioblastoma;Hippo,NF-?B,TNF and other signaling pathways may be important pathways for AEBP1 overexpression to promote the development of glioblastoma.AEBP1 may be a potential target for the treatment of patients with glioblastoma. |
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