The Pathogenesis Of Neuroendocrine Tumor Of Small Intestine Based On Bioinformatics Investigate

Objective: The pathogenesis of neuroendocrine tumor of small intestine based on Bioinformatics investigateMethod: THE microarray data set(gse65286)of gene expression synthesis(GEO)database was analyzed Rstudio tool was used to screen differential expression genes(DEG)between Nen and normal tissue.Use note interpretation visualization and intergrated,Discovery database perform gene ontology functions and the whole genome of the Kyoto protocol and gene pathway enrichment analysis to identify the pathway and functional annotation of deg.the protein-protein interations of these DEG were analyzed based on the search tool of searching interaction gene database,and visualized by the software of cytoscape.In addition,we sued GEPIA for survival analysis of HUB gene to evaluate the prognostic value of HUB gene expression in patients with small intestinal neuroendocrine neoplasm.Results: GSE65286 data set(including 10 normal small intestinal neuroendocrine neoplasm10 primary small intestinal Nen tissues and 23 distant metastasis tissues)was obtained from Geo database.Rstudio was used to analyze gse65286 data set and screen the differential expression gene(DEG)between small intestinal neuroendocrine neoplasm and normal tissues.658 differential genes was obtained,there are 314 up-regulated genes and 344 down-regulated genes.In order to identify the approach and functional annotation of DEG,we used David to analyze the enrichment of DEG by GO and KEGG.The proteinprotein interactions of these DEG were analyzed based on the search tool of searching interaction gene database,and the first ten genes were evaluated by the connectivity degree of PPI network through the software of Cytoscape.The results showed that 5 of F2.ALB.CASR.TAC1.FGG were up-regulated genes and 5 of CG.APOA1.LPAR5.NMU.CCK were down-regulated genes.In addition,we used GEPIA to conduct survival analysis of HUB gene to evalutate the mall prongnostic value of central gene expression in patients with neuroendocrine neoplasm.Conclusion: the abnormal expression of TAC1 ALB FGG LPAR5 is related to the poor clinical result of patients with intestinal neuroendocrine neoplasm.In Si-nens patients,overexpression of TAC1 is an unfavorable prognostic factor.These core genes may be potential makers for improving diagnosis,optimizing chemotherapy regimen and predicting prognosis.in addition,pathways related to these genes may be potential therapeutic targets for Si-NENs.