Study On The Separation And Preparation Of Hypoglycemic Active Compounds From Potentilla Fruticose.L

Potentilla fruticose L.is a Potentilla deciduous shrub of the Rosaceae family,also called Potentilla Mela,Potentilla tea,Potentilla flax and Wintersweet.This paper study on the chemical ingredients in potentilla fruticosa basis using column chromatography,high performance liquid chromatography?HPLC?,BUCHI fast optimization system of three kinds of separation methods such as preparation of potentilla fruticosa compounds has hypoglycemic activity of the compounds,and the alpha glycosidase inhibitor activity were determined,and committed to the technology research of potentilla fruticosa material standard,for the separation of the standard preparation of potentilla fruticosa offer feasible method,at the same time as the research potential of safe and effective to provide theoretical support for the prevention and treatment of diabetes drugs.The main research contents are as follows:The main research contents are as follows:1.Potentilla fruticosa L.pieces,precision according to take 500 g,and g/mL and solid-liquid ratio,75%ethanol,80?reflux extracting 3 times,each time 2 h,merge the filtrate,crude extract was obtained,0.6 L,in turn,with 10 L volume of quality score of 0,10%,20%,30%,40%,50%,60%,70%,80%methanol elution,containing 20%of the target compounds was obtained elution,24.7019 g.2.Richment containing a target component through the preparation of high performance liquid chromatograph?DAC-50?,the chromatographic column for the DAC-HB50,column temperature 30?,45%methanol,such as degree of elution,flow rate of 50 mL/min,detection wavelength of 254 nm and 280 nm,sample quantity3 mL,sampling interval 50 min,adopts repeated sampling method,separation,preparation of get target compounds Quercetin-7-O-?-D-glucuronide?compound 1?,the quality of 2481.8 mg,The purity was 97.9516%.3.Through a rapid purification system?BUCHI Peveleris Prep?,a preparative column was used:Jiangyin hanbang Dubhe C18?10 mm×250 mm,5?m?,column temperature 30?,20%methanol elution,flow rate of 50 mL/min,detection wavelength of 254 nm and 280 nm,sample volume of 1.5 mL,sampling interval is 30min,adopts repeated sampling method,separation of Gallic acid preparation to get the target compounds?compound 2?3,4,6-tri-O-galloyl-?-D-glucose?compound3?,quality were 18.6 mg and 253.8 mg,The purity was 95.7021%and 96.1672%,respectively.4.By measuring the inhibitory activity of AG,the maximum inhibitory rates of compound 1,compound 2 and compound 3 were 94.672%,67.195%and 96.340%respectively,and the IC₅₀0 of compound 1,compound 2 and compound 3 for AG were0.259,0.831and 0.021 mmol/L.Compound 1 was a competitive inhibitor.Compound 2is an anti-competitive inhibitor.Compound 3 was a non-competitive inhibitor compound 1,compound 2 and compound 3 all significantly inhibited the activity of AG.