Diversity And Community Structure Of Bacterial In The Lakeside Soils Of Baiyangdian

The riparian zone soil has unique geographical and geomorphological conditions,and the microbial population formed is also relatively unique.The diversity of microorganisms and the community structure are also more likely to be affected by human interference.In this study,the bacterial diversity of 6 sampling points(S1: Aquaculture area,S2: Residential living area,S3: Still water accumulation area,S4: Representative lake,S5: Tourist area,S6: Suburban pollution-prone area)representing different interference conditions and functional areas in the shoreline of Baiyangdian was studied.Through Illumina Miseq highthroughput sequencing technology and the determination of soil environmental factors,analyzed and compared the bacterial community structure and diversity distribution characteristics in different areas of the lakefront zone and in different functional areas.Simultaneously used a variety of media to isolate the bacteria in the sample and studied the diversity of bacteria that can be cultivated.The results were as follows:(1)In the six areas with different characteristics in Baiyangdian,the bacterial communities have high diversity,the species composition and relative abundance of the samples are different,and the proportion of dominant species is slightly different;The inter-group differences of the 6 sample grouping communities were significantly larger than the intra-group differences,indicating that the bacterial community will change with the characteristics of Baiyangdian area.Species abundance has a significant effect on sample differences.Proteobacteria,Nitrospira,Phytophthora,and Phytophthora have significant differences among the samples.There are many links between the bacteria in the 6 regions,which can affect each other's species abundance.Among these samples,the bacterial diversity of S1 and S6 is similar,the bacterial diversity of S2 and S3 is similar,and the bacterial diversity of S4(Representative lake)is significantly different from all samples.The microbial metabolic function in the sample is very rich,but its abundance of KEGGPathway shows a certain difference from other samples: lower environmental information processing and higher metabolism.(2)The environmental factors of the six soil samples are quite different.S1 has higher contents of heavy metals,polycyclic aromatic hydrocarbons,phthalates,total phosphorus,total nitrogen,and organic matter,followed by S6,however,the S4 organochlorine pesticides are significantly higher than in other regions.Environmental factors such as Zn,As,Mo,V,Sb,naphthalene,fluoranthene,?-666,?-666,?-666,TP,OM,and p H all explain the differences in grouping,among which Zn,Sb,and V may be the main reasons for S1 and S6 to cluster on the bacterial community structure,Mo,As,and p H may be the main reasons for S2 and S3 to be clustered on the bacterial community structure,and S4 and other samples the large difference in community distribution may be affected by OCPs.There is a strong correlation between environmental factors and species.Mo>Sb>dibutyl phthalate>As>fluoranthracene are positively related to environmental factors and species,and p H>?-666=?-666=?-666>OM>Diisobutyl phthalate are negatively related to environmental factors and species.(3)A total of 113 strains of bacteria were isolated in this experiment,incloud 49% Proteobacteria,30% Pachyphyta,18% Actinomycetes,3% Bacteroides.The species and number of Streptomyces,Bacillus,and Pseudomonas are dominant.The dominant bacteria are different in different samples,and the bacterial diversity of S4 and S5 samples is better.The strain HBUM207130 isolated from sample S4 is Pseudarthrobacter sp.In the first two chapters of culture-free analysis,it is speculated that Pseudarthrobacter sp.in the S4 area may have higher pesticide degradation activity,and its pesticide degradation activity can be further explored in the future.The 16 S r RNA gene sequence similarity between the isolated bacteria(HBUM207136,HBUM207145)and the closest model bacteria is less than 98%,which may be a potential new species.