Application Of Dual-enzyme Nanoflower In The Epoxidation Of Alkenes And The Amidation Of Anilines With1,3-Diketones

The catalytic promiscuity of enzymes has been reported with the development of enzymology.Lipase has been widely used for organic synthesis reactions because of the ability of an enzyme of catalyzing completely reactions relative to the natural reaction.The use of industrial catalyst led to environment pollution.As an important oxidase,lipase's biologic role as a catalyst is important,so its basic research has important significance and great business potential.Over the past decade,multi-enzymatic cascade reactions have been become an important and popular topic in biochemical research Such reactions offer considerable advantages,including the regeneration of cofactors,shifting reaction equilibria,and overcoming inhibition effects as well as reducing the reaction time,energy,and material demands of a given process.Generally,multi-enzymatic cascade systems must be robust and recyclable for practical application.Coimmobilization is a key technique to increase the operational performance of enzymes and,more importantly,to enhance the catalytic activity of enzymes in multi-enzymatic reactions.In addition,the stability and reusability of coimmobilized multi-enzymes have attracted interest from an environmental and economic point of view.In this study,a novel dual-enzyme nanoflower was successfully prepared for the coimmobilization of GOx and lipase and used in the epoxidation of alkenes and amidation of anilines with 1,3-diketones.In this nanoflower,cupric phosphate was used as the inorganic component,while glucose oxidase from A.niger and lipase B from Candida antarctica(CalB)were adapted as the organic components to construct the dual-enzyme nanoflower.The dual-enzyme nanoflower was characterized by scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FTIR)and then used in the epoxidation of alkenes and amidation of anilines with 1,3-diketones,for which reaction conditions were optimized.Compared with the free dual-enzyme,the dual-enzyme nanoflower exhibits a much higher catalytic performance in the epoxidation of alkenes and amidation of anilines with 1,3-diketones,and excellent reusability,which was observed in this reaction system.Moreover,this study provides a new case of multienzyme coimmobilized organic-inorganic hybrid nanoflowers for multistep cascade enzymatic reactions.