Highly Efficient Solid-phase Labeling Of Saccharides And Molecular Imprinting Of Amadori Compounds Based On Mesoporous Silica Nanoparticles

Saccharides,as essential components of all living systems,are the major compounds of interest in many fields such as biochemistry,biomedicine,glycomics and glycobiology.The complexity of the saccharides structure,leads to the lack of effective means of sequencing.Meanwhile,the content of saccharides from biological source are low and since biosynthesis of saccharides does not rely on any template,so it can't be amplified by similar PCR or molecular cloning.As the result,in separation and purification of oligosaccharide chains,the requirements for detection technology must possess high sensitivity.Due to the diversity and complexity of the structure,as well as the limitations of current analysis method,makes the study of saccharides is lagging in nucleic acids and proteins.It is a big challenge for life science to do scale and through quantitative research of saccharides like genomics and proteomics.So,high selectivity and high enrichment of saccharides analysis method is necessary.Saccharides have no fluorescence,limited UV-vis absorbance and poor mass spectrometric(MS)signal,labeling with an appropriate signal-producing reagent is thereby a critical step prior to their detection,quantitative analysis and structural identification.The labeling is usually carried out in liquid phase.However,it is associated with apparent disadvantages,including time-consuming,the presence of excessive labeling reagent,and higher concentration requirement of the saccharide to be labelled.Therefore,new labeling approaches that can overcome these drawbacks are of significant importance.In this article,on the basis of boronic acid-functionalized mesoporous silica nanoparticles,on the one hand,we present a solid-phase approach for highly efficient labeling of saccharides,using boronic acid-functionalized mesoporous silica nanoparticles(MSNs)as selective extraction sorbent as well as nanoscale reactor.The solid-phase labeling approach exhibited several significant advantages,including:1)much faster reaction speed(taking only 2 min),2)high product purity,and 3)much lower applicable saccharide concentration(4 orders of magnitude lower than that of liquid-phase labeling).Thus,this labeling approach opened up new avenues to the facile and efficient labeling of saccharides.On the other hand,based on molecule imprinting technology and the principle that confinement effect can reinforce intermolecular force,we design and synthesized mesoporous silica based molecular imprinting material by using Glu-Trp as the template.Because of the confinement effect provided both by mesopores from the material and nanopores from the imprinting site,the obtained material exhibits good selectivity and high affinity to the target molecules and can extract the target from complex samples(tobacco).Nano imprinting cavity provides new ideas for the development of molecular imprinting technology.