Evaluation Research Of Antioxidant ?hypoglycemic And Hypercholesterolemia-induced Of Flavonoids From Sorbus

Objective:This article is Sorbus antioxidant flavonoids and lowering blood sugar,lowering blood pressure to evaluate the function of research.Antioxidant capacity of the tumor suppressor role of the cellular level through research rowan flavonoids in vitro,the last type 2 diabetes in vivo experimental model induced by high glucose and high fat diet mice,etc.,to evaluate and explore Sorbus flavonoids lower blood sugar,blood fat role in providing a theoretical basis for further study on the pharmacological effects of flavonoids rowan Sorbus series and product development.Methods:Ultrasonic assisted extraction of total flavonoids were isolated Sorbus extracted using rutin standard curve to determine the content of total flavonoids Sorbus;using three macroporous resin(X-5,D101,AB-8)of Sorbus flavonoids crude extract was purified Comparison of three macroporous resin adsorption rate and desorption rate,choose a suitable resin as a test of the pre-resin.Determination of the use of thiobarbituric acid(TBA)anti-lipid peroxidation;use a solution of potassium hexacyanoferrate(K3Fe(CN)6)Determination of reducing power;measured by the pyrogallol method superoxide anion radicals(O2-�)the ability and determination of salicylic acid scavenging hydroxyl radical(� OH)are.HEK293 cells using genomic DNA,using the Fenton solution damage,repairing the protective effect of flavonoids on Sorbus reactive oxygen species induced by DNA damage.At the same time,the use of bovine serum albumin damage Fenton solution,research Sorbus flavonoids on the protein induced by reactive oxygen species damage repair protection.Sorbus BGC823 flavonoids on cell inhibition was measured by MTT assay.Male mice,using STZ type 2 diabetic mice,blood and removal of the liver,serum of TC,TG and HDL-C index,and liver histopathological examination.The result:(1)According to rutin standard curve y = 12.134 x + 0.0015,R2 = 0.9996,Sorbus Determination of total flavonoids in the sample was 0.74%.Select AB-8 macroporous resin purification Sorbus flavonoid crude extract,the adsorption rate of 61.9%,analytical rate of 89.1%.After freeze-drying,flavonoids have not been compared with AB-8 macroporous resin purification,the content from 8.4% to 32.4%.(2)Sorbus fruits of total flavonoids of Fe2+ induced lecithin Lipesome peroxidation was significantly better than ascorbic acid,when the sample concentration of 600?g/ml,the inhibition rate reached 49.66%;reducing power of the samples increases with the concentration of increases;when the sample concentration of 300?g/mL,superoxide anion radical scavenging capacity has reached 83.33%;at the concentration of 200?g/mL,the hydroxyl free radical scavenging rate of 60.49%,indicating Sorbus Flavonoids have strong antioxidant capacity.Sorbus protective effects of flavonoids damage to DNA and protein increases the concentration of flavonoids Sorbus effect is more obvious,when the concentration of flavonoids Sorbus 5mg/mL and 10mg/mL,the protective effect on DNA damage is more significant.At a concentration of 10mg/mL,the protective effect of BSA protein strongest.Sorbus flavonoids on BGC823 cells proliferation was inhibited,in 24 to 48 hours over time increase the inhibition rate increases during 48 h,the inhibition rate reached 50%.72 h may be due to cell density is too large,affecting cell growth,and therefore no significant time variation.(3)with high glucose and lipid group(HFC group)compared Sorbus flavonoids,the high-dose group can significantly reduce body weight and liver weight in mice,and significantly increased with the dose enhancement;Sorbus flavonoids can significantly reduce small Rat blood sugar,while lowering serum TC and TG levels,increase HDL-C levels.Compared with the HFC group fed with increasing dose,serum TC and TG mice was significantly decreased,while HDL-C concentrations were significantly increased.Conclusion:The results of the study showed that the flavonoids Sorbus has strong antioxidant capacity,capable of oxidative damage to DNA and proteins and other biological macromolecules protect or repair effect,but also the value of gastric cancer cells was inhibited.Sorbus flavonoids can reduce serum lipids and glycemic index content in the blood and reduce lipid accumulation,achieve the aim of improving lipid metabolism disorders.And,Sorbus flavonoids as a new raw material,anti-oxidant and tumor suppressor,regulating the development of health food products,such as lipid metabolism has great application prospects.High-salt diet is an important external factor that threatens human health,which can lead to high blood pressure.It closely associated with cardiovascular disease.Increasing level of serum homocysteine(Hcy)causes Hyperhomocysteinemia(Hhcy).Many studies have shown that Hhcy is an independent risk factor for cardiovascular disease.High-salt diet and Hhcy are associated with cardiovascular disease,but there is little research on the relationship between the high-salt diet and Hhcy.This study devoted to exploring the effects of high salt stress on the metabolism of Hcy at animals and cellular level.Betaine and taurine,which are osmotic pressure regulators and related to Hcy metabolism,are selected to research whether they antagonistic high salt stress on Hcy metabolism.Studies provide theoretical guidance for further study the relationship between high-salt diet and Hhcy Hhcy and prevention and treatment.