Ephedran The Study Preparation Technology And Quality Control

Ephedra(Ephedra sinica Stapf)has a long history of application,described in the beginning of "Shen Nong's Herbal Classic",the sweating of the strong,the Xin Wen Jie Biao first to the drug,and therefore commonly used in clinical cold,cough,shortness of breath,swelling of feng shui,Bronchial asthma embolism treatment.As recent studies have found that Ephedran is one of the main active ingredient of ephedra,the chemical composition of ephedra Our previous polysaccharides and biological activity results show Ephedran immunosuppressive,which has a production of drugs broad prospects for development,so that the production process simplification and practical operability,on this basis,this paper carried out the following experiment.For the preparation of this paper,the process is preferably Ephedran,using different time ethanol precipitation,ethanol precipitation purification Ephedran different times,ethanol and 90%ethanol washing method washing method,tested Ephedran preferred production process for the extraction and purification of two alcohol precipitation law,namely an increase of 10 times the amount of water,extracted three times,each time 3 h,plus 95%ethanol containing alcohol amounted to 75%,alcohol quiet only 24 h,Ephedran was dissolved in distilled water,and then 95%ethanol alcohol precipitation making alcohol content of 75%,still 24 h,filtration,the precipitate was collected and dried to obtain Ephedran.Phenol-sulfuric acid method results Ephedran Determination of total sugar,two alcohol precipitation method P1,P2,P3 total sugar content obtained were as follows:68.7%,71.2%,67.7%;with Coomassie brilliant blue Determination of protein Ephedran,results of the two alcohol precipitation method P1,P2,P3 protein obtained were as follows:7.22%,5.33%,6.60%;Measurement Ephedran of ephedrine and pseudoephedrine using high performance liquid chromatography content was twice the alcohol precipitation method P1,P2,P3 ephedrine were obtained:0.0193%,0.0201%,0.0207%;pseudoephedrine content were:0.00614%,0.00348%,0.00552%;the second derivative method using spectrophotometry Determination of the total alkaloids Ephedran,the result of a alcohol precipitation method P1,P2,P3 total alkaloid content obtained were as follows:0.046%,0.045%,0.047%,twice the alcohol precipitation method P1,P2,P3 obtained The total alkaloid content were:0.0318%,0.0295%,0.0328%;Measurement Ephedran both Polysaccharide ESP-B4 using HPLC-ELSD method,the results of the two alcohol precipitation method P1,P2,P3 are obtained polysaccharide ESP-B4 contents were:32.4%,30.9%,29.3%.By content Ephedran compare different methods of extraction and purification of the resulting component of the index shows that the two extraction alcohol precipitation Ephedran high efficiency,good impurity effect,safe,simple,stable and easy,there are prospects for industrial application,suitable for industrial production,while providing reference and scientific basis for further research and product development Ephedran of.