Isolation&Purification Of Ophiopogonin D And Ophiopogon Polysaccharides

In this paper,saponins and polysaccharides were extracted from Chinese medicine roots of Ophiopogon japonicus,and then the ophiopogonin D and other ophiopogonins were separated and purified by silica gel column.And to getting low molecular weight ophiopogon polysaccharides,the alkaline and enzyme hydrolysis on the polysaccharides from Ophiopogon japonicus were studied.Total ophiopogon saponins was extracted from the roots of Ophiopogon japonicus.18.0 g total ophiopogon saponins was extracted from 10 kg roots of Ophiopogon japonicus by methanol,the yield was 0.18%,and then 18.0 g total ophiopogon saponins was separated by silica gel column to obtained ophiopognin D 2.31 g,the ophiopognin D yield was 12.8%for total ophiopogon saponins.The purity of ophiopogonin D was not less than 91.2%by the method of HPLC.When the Absidia sp.o84s strain was fermented in the medium containing with 20%Ophiopogon japonicus as inducer at 30 ℃ the enzyme hydrolyzing the polysaccharides from root of Ophiopogon japonicus was good produced.The enzyme optimal reaction condition was:1%concentration substrate,pH 5.0,40℃ react for 60min.Viscosity analysis was used for determination of the average molecular weight of ophiopogon polysaccharides.The average molecular weigh of ophiopogon polysaccharides extracted by water was 62000;which extracted by weak alkali was 41000.After alkaline hydrolysis,the average molecular weight of ophiopogon polysaccharides was 35000;its enzymic hydrolysates was 32000 after the enzyme fermentation,and its enzymic hydrolysates was 28000 after the enzyme reaction,the experience constant was K = 1.34 × 10-3.