The Research Of Lovastatin And Ergosterol Of Monascus Products

Monascus is able to produce a variety of functional metabolites,which have different value and application prospects.The Lovastatin and Monacolin K are two kinds of natural products produced by Monascus in the process of growth and metabolism,having a cholesterol-lowering effect.Ergosterol is one kind of natural product produced by Monascus in the process of growth and metabolism,having the effect of preventing osteoporosis.China is a major producer of the red yeast,distributing in fujian,zhejiang,guangdong,jiangsu,Taiwan,and other provinces.Fujian province is China's traditional production of red kojic rice main production,red kojic rice production and exports have been in the domestic leading position for a long time,more than half of The national.Therefore,further research and development of the functional components of the red yeast rice has a very important significance.This article use solid-state fermentation culture and high performance liquid detection,investigating the extraction methods,strains,culture conditions and medium ratio of metabolites.the main findings are as follows:1)By screening strains wo found strain B2M2 yielding lovastatin and monacolin K and strain F6B2 yielding lysergic steroids.2)Compare different extraction solvent on effect of lovastatin ang monacolin K,methanol is best.3)Use analytical method of HPLC-DAD,maximum absorption wavelength of lovastatin and monacolin K is 238nm,column is Xtimate C18(4.6 × 250mm;5?m),mobile phase is acetonitrile—0.1%H3P04(75:25),velocity of flow is 1.5ml/min.the column temperature is room temperature,injection volume is 20?l.The method has a good linear relationship,separation and very good reproducibility.4)Use 3 factor quadratic general rotary unitized test design,Get solid-state culture monascus products,monacolin K(Y1),lovastatin(Y2)and total(Y3)for glycerol concentration(X1),the peptone(X2)and trisodium citrate concentration(X3).5)Ergosterol use high performance liquid detection,The standard curve have a linear relationship within the scope of 0.01 mg/ml and 0.8mg/ml.Regression equation of ergosterol is y = 37943x +248.22.R2 =0.9974.Precision experiments RSD is 0.22%and the experimental reproducibility RSD is 0.22%,recovery rate is 98.82%.The method is stable,simple and accurate.6)Use 3 factor quadratic general rotary unitized test design,Get solid-state culture Monascus products,Ergosterol concentration Y for magnesium sulfate concentration(X1)lactose concentrations(X2)and beef extract concentration(X3).