Fuzhuan Instant Tea Regulates Lipid Metabolism And Intestinal Flora In High-fat Diet Mice

Fu brick tea is a unique type of black tea.The liquor color is bright and dark reddish and the liquor tastes smooth and refreshing.Fu brick tea is known to have good lipid-lowering,liverprotecting effects and cleansing intestines.Instant teas are convenient to drink,easy to store and transport,and preserves the original flavor and quality of tea to the greatest extent It has been loved by consumers in recent years.In this study,by comparing the effects of different processing methods on the effective ingredients and flavor of the tea,the preliminary research on the production and processing methods of tea made from tea bricks;The mechanism of action,while exploring the regulation of FBIT on the intestinal flora and short-chain fatty acids of diet mice,providing a theoretical basis for the production and development of FBIT,the specific research results are as follows:(1)The powder yield is about 15.8%by using micro-membrane filtration plus spray drying.The micromembrane filtration plus freeze-drying yielded a powder yield of 19.2%,and the coarse filtration plus freeze-drying yielded a powder yield of about 22.4%.Micro-membrane filtration has better retention of tea polyphenols and theanine,freeze-drying has better retention of caffeine,and micro-membrane filtration plus freeze-dried instant tea tasted best.However,the cost for freezedrying is too high and takes too long.Direct coarse filtration saves time but has more impurities.Based on comprehensive consideration,we conclude that the micromembrane filtration in conjunction with spray drying method is most suitable for the instant tea production from Fu brick tea.(2)FBIT significantly inhibited the weight gain of the high-fat diet mice after 9 weeks of feeding trial without affecting the feed and water intakes.Compared with the high-fat group,the average weight of the high-fat diet plus tea gavage group decreased by 15.8%.At the same time,FBIT repaired the abnormalities of TC,TG,LDL-C,and HDL-C in blood lipids caused by high-fat diets,reduced fat accumulation,organ enlargement,reduced AST and ALT levels in serum,and reduced liver damage in mice.(3)At the gene level,FBIT down-regulated the expression of genes,such as ACC and FAS,that regulate TG synthesis in high-fat diet mice,up-regulated the expression of LPL,CPT-1,and other genes that regulate ?-oxidative oxidation of fatty acids,down-regulated the expression of PPARy genes,reduced the differentiation of preadipocytes.FBIT feeding also down-regulated the expression of SREBP-1C gene and up-regulated the expression of LxRa gene,reduced the synthesis of TC and accelerated the breakdown of TC.At the protein level,FBIT down-regulated the level of ACC,FAS,PPARy,and other proteins,increased CPT-1 protein level,reduced fatty acid synthesis and new adipocyte formation,and accelerated fatty acid oxidation.FBIT also decreased SREBP-1C protein level and reduced cholesterol synthesis.(4)Through high-throughput sequencing,FBIT can effectively regulate the intestinal flora composition of high-fat diet mice,increase the diversity of intestinal flora,increase the relative abundance of Bacteroides,and reduce the relative abundance of Firmicutes.Increase Lachnospiraceae NK4A136 group,Ruminiclostridium 9,Bacteroides and other bacteria with a positive correlation with SCFA content abundance,to a certain extent restore the decline in intesstinal SCFA content caused by high-fat diet,improve the intestinal acetic acid,propionic acid,butyric acid,pentyl Content of SCFA such as acid At the same time,the rise of SCFA content has strengthened the acidic environment in the intestine,better promoted the growth of beneficial bacteria in the body,and improved the intestinal flora imbalance caused by a high-fat diet.