The Design And Synthesis Of Novel Rhodamine Fluorescent Probes And Their Detection Of Metal Ions Research

Metal ions play important roles not only in chemical reactions but also in a lot of biological processes.They are indispensable in our daily life.As a result,it is necessary to find efficient ways to detect different ions selectively and sensitively.Fluorescent probes have been designed and synthesized to meet the above acquirements.Fluorescent chemosensors now are widely used in medical,environmental,and biological applications.They can show scientists the molecular details of biological processes involved in the change of metal ions.Chemosensors can make it possible for remote measurement,hence avoiding the risk of damaging the samples.For the above reasons,the development of selective and sensitive chemosensors has drawn a great deal of attention.This dissertation described the design and synthesis of 3 rhodamine amide derivatives,the recognition performance in aqueous solution,response mechanism and actual application were investigated.1)A new rhodamine derivative?FP1?with a recognition capability for Fe³⁺was synthesized and employed as a fluorescent probe for Fe³⁺in real water sample.The spirolactam ring-opening of probe FP1 induced by Fe³⁺lead to a highly selective fluorescence“turn-on”response toward Fe³⁺over other metal ions with micromolar sensitivity.The 1:1 stoichiometric structure between FP1 and Fe³⁺were supported by Job's plot.The analytical results obtained by fluorescence spectrophotometry show that the linear range and the limit of detection?LOD?of the present probe for Fe³⁺are40-200 and 0.17?M,respectively.The present probe was successfully applied to the detection of Fe³⁺in real water sample.2)A new rhodamine derivative?FP2?with a recognition capability for Fe³⁺was synthesized and employed as a fluorescent probe for Fe³⁺on steel sheets.The spirolactam ring-opening of probe FP2 induced by Fe³⁺lead to a highly selective fluorescence“turn-on”response toward Fe³⁺over other metal ions with micromolar sensitivity.The 1:2 stoichiometric structure between FP2 and Fe³⁺were supported by Job'splot.TheanalyticalresultsobtainedbyUV-visandfluorescence spectrophotometry show that the linear range and the limit of detection?LOD?of the present probe for Fe³⁺are 30-150 and 0.31?M,respectively.The present probe was applied to the detection of Fe³⁺on steel sheets.3)A new rhodamine derivative?FP3?with a recognition capability for Fe³⁺was synthesized and employed as a fluorescent probe for Fe³⁺.The spirolactam ring-opening of probe FP3 induced by Fe³⁺lead to a highly selective fluorescence“turn-on”response toward Fe³⁺over other metal ions with micromolar sensitivity.The 1:4 stoichiometric structure between FP3 and Fe³⁺were supported by Job's plot.The analytical results obtained by UV-vis and fluorescence spectrophotometry show that the linear range and the limit of detection?LOD?of the present probe for Fe³⁺are 100-500 and 0.39?M,respectively.