Preparation Of Self-regulating/Anti-adhesion Hydrogel And Study Of Its Performance To Promote Healing In Burn Wound

Burn wounds occur widely,causing serious damage or loss of skin tissue that is essential to the human body.Therefore,it is still necessary to identify suitable burn dressings to control wound infection and promote healing in burn management procedures.Dressings for different types of wounds face different challenges at different stages of the wound healing process,so an ideal burn dressing should exhibit multiple characteristics.Although there are many different types of dressings under development and on the market,none of these dressings currently fulfil all of the characteristics required of an ideal burn dressing.The most important features of burn wounds are easy to scab,adhere to dressing,easy infection and difficult to heal,etc.So the development of dressings for solving these problems is a major and urgent keynote.Therefore,the aim of this study is to develop a burn dressing that can self-regulate the optimal humidity levels that are required for wound healing,whilst also providing good anti-adhesive properties when dressings are change.Ultimately,it is necessary to promote burn wound healing effectively.Based on this,this study has involved the thermal bonding technology to prepared a water-soluble hydrogel as a wound dressing that is derived from a water-soluble matrix [carboxymethylcellulose sodium(CMC-Na)/ sodium alginate(SA)/ Propylene glycol(PG)] that has been incorporated into a chitosan(CS)matrix used a high-speed blending process.Orthogonal testing revealed an optimal ratio of CMC-Na,SA and CS as 2%,3% and 1% in 1% PG aqueous solution for hydrogel preparation with optimal properties of self-regulating/anti-adhesive,respectively.Combined with analysis of macroscopic observation,microscopic observation and Fourier Transform Infrared Spectroscopy,the composite wound dressing prepared from this optimal hydrogel has exhibited good water vapor permeability properties that suitable for the healing of deep second degree burn wounds.The cytotoxicity of SR-AA dressing extraction on L-929 and HSF in vitro showed that it significantly promoted cell growth in the early stage of cell growth,whilst significantly inhibited the overgrowth of cells in the later stage,and cytotoxicity rating showed that it had good cytocompatibility.At the same time,the response of the SR-AA dressing to the skin and the evaluation of blood compatibility were all in accordance with the safety standards of biomaterials.Finally,combined with analysis of wound morphology,wound healing rate,staining for wound tissue,the dressing showed excellent anti-adhesive performance and significantly promoting healing when it was used for the treatment of deep second degree burn wounds in SD rats.The pro-healing mechanism of the dressing has been investigated,which revealed that VEGF expression was up-regulated and bFGF expression was down-regulation in the early periods of wound healing to promote angiogenesis,with up-regulation of b FGF then occurred at a later stage of wound healing to promote regeneration of epithelial cells.At the same time,the wound dressing resulted in decreased levels of TNF-? and IL-6,thus validated its beneficial effect on the wound healing process at a biomolecular level.In conclusion,this new hydrogel dressing was shown to excellent self-regulatory and anti-adhesive properties that synergistically promote the healing of burn wounds in rats,which was providing promising results that may have clinical applications,whilst bring gospel to burn patients and burn management procedures.