Fast Detection Of Escherichia Coli In Food Using Nanoprobe And ATP Bioluminescence Technology

In recent years,with the rapid development of our economy,food and clothing are no longer the most important problem,while the food safety has become the focus.People pay more attention to food hygiene today,at the same time the traditional detection methods have been unable to meet the needs of the development of modern society,people put forward higher requirements on the detection technology,which makes the detection more accurate and more accurate.It has become a hot research topic.Nano probe technology is relatively hot of the sensor technology,it can be widely applied in antigen antibody detection,identification and genetic engineering of microorganisms.Its operation scale is at the nanoscale level,which can reach the single cell level and greatly improve the accuracy of detection.Magnetic nanoscale probes play an important role in the recognition and enrichment of microbes.ATP bioluminescence technique is a frontier method to detect the number of bacteria,the use of ATP is widespread and special luminescence mechanisms can be used to detect the number of some microorganisms,the method greatly reduces the detection time which can obtain the results in twenty minutes,and has wide application prospect.In this paper,a biosensor system based on nano magnetic probe technology and ATP bioluminescence technique to detect the number of colonies of Escherichia coli in food is proposed.In the design,we use the nanomaterials with special antibodies on the surface as probes to capture Escherichia coli through specific binding between antigen and antibody,and finally enrich the target bacteria by external magnetic field.After the detection of ATP or a luminous experimental equipment,automatic adding reaction process is controlled by MCU peristaltic pump reagent,the photomultiplier converts the optical signal into electrical signals,and then processed module can detect quickly the number of Escherichia coli and the signals through the signal processing,the test time can be less than twenty minutes.Detection of bacterial concentration range of 10²¹0⁸ CFU ml^-1.Under the optimal test conditions,the detection limit?LOD?is 3×10² CFU ml^-1.The data of independent experimental measurements show that the maximum wave-to-noise ratio is less than 6.98%,which shows that the experimental design has good reliability and repeatability.The signal processing module includes a differential amplifying circuit.The amplification factor is about 3000 times,and the SNR of the signal to noise ratio is less than 50 dB.In addition,the linear correlation coefficient of the experimental data is 0.964.It can be seen that the measured performance of the system is good.This topic describes the development process of nano probe technology and ATP bioluminescence technology,as well as the development status at home and abroad.Combined with the advantages of the two technologies,it has achieved good results by complementary integration.