Composition Analysis And Properties Of Turmeric Volatile Oil

Turmeric(Curcuma longa L.),as a kind of traditional perennial herb has been used both as medicine as well as food,and is widely cultivated in southeastern and southwestern part of China.It widely applied in the field of traditional Chinese medicine with the function of adjusting menstruation and analgesia,dispelling wind and activating blood and fending off the cold and anti-inflammatory.As a major active ingredient in turmeric,turmeric volatile oil is a kind of natural pigment and has been used in the food industry with good dyeing effect and high security.Due to its properties of anti-oxidation,anti-cancer and scavenging free radicals,curcuminoid has been broadly applied in clinic as anti-cancer drugs,and shows good anti-oxidation and whitening effect in the cosmetics industry.Recently,more and more studies of turmeric volatile oil are applied to health foods.This paper was mainly to study the extraction method and main components of turmeric volatile oil from the rhizome of turmeric,and discuss the antioxidation,antibacterial,anti-inflammatory and analgesic activities and anticancer activity of volatile oil from turmeric.The main research contents and results are as follows:Firstly,taking turmeric rhizome as raw material,Chinese Pharmacopoeia method was used to detect turmeric samples from different producing areas and years,and suitable experimental samples were selected.And established a new extraction method,the ionic liquid [BMIM]PF6 enzyme method: For this purpose,a regression model was developed to describe the yield of curcuma oil as a function of 4 independent variables including ionic liquid concentration,cellulose concentration,liquid-to-solid ratio and hydrolysis temperature.The optimum extraction parameters were determined as follows: ionic liquids concentration,18%(V/V);cell?Lase concentration,1.4%(m/m);liquid-to-solid ratio,10:1(m L/g);and hydrolysis temperature,50?.Under these conditions,the yield of curcuma oil was 5.0 m L/g.The essential oils obtained from SD and ILSE were analyzed by gas chromatography-mass spectrometry(GC-MS).A total of 48 and 53 components were identified,respectively.Compared with the steam distillation method,the volatile oil component of turmeric obtained by the ionic liquid enzymatic method increased.The volatile oil components of turmeric extracted by the two extraction methods are mostly sesquiterpenes,and the main components are flavonoid flavonoids,curcumin and so on.Secondly,The obtained turmeric volatile oil was treated by in vitro sim?Lated digestion to obtain simulated gastrointestinal digestion samples,respectively.Through the four aspects of DPPH free radical scavenging ability,total antioxidant capacity,superoxide anion scavenging effect and antioxidant capacity in edible oil,comprehensive evaluation of the anti-oxidation ability of turmeric volatile oil.The results show: The gastrointestinal digestive liquids of turmeric volatile oil and turmeric volatile oil all had good antioxidant effects and depended on dose.Among them,the total antioxidant effect of turmeric volatile oil is the best,with an EC50 value of 0.75 mg/m L.The anti-oxidation effect of turmeric volatile oil after digestion by gastrointestinal fluid was higher than that of turmeric volatile oil,which proved that the volatile oil of turmeric after digestion was more conducive to human body's elimination of free radicals.In addition,the EC50 value of DPPH radical and total antioxidative activity in the intestinal fluid of turmeric volatile oil was lower than that of VE gastrointestinal fluid,indicating that the antioxidative effect of turmeric volatile oil intestinal fluid was also higher than that of VE gastrointestinal fluid.Thirdly,In order to study the antibacterial activity of turmeric oil,the inhibition zone experiment on Bacillus subtilis,Bacillus cereus,Rosa m?Ltiflora and Salmonella was used to determine the minimum inhibitory concentration(MIC).The mechanism of antibacterial activity was also studied by measuring the growth curve,conductivity,content leakage and cell morphology of four tested bacteria.The results showed that turmeric oil had antibacterial effect on four kinds of test bacteria,but stronger against Salmonella(The inhibition zone was 15 mm,the MIC value was 0.4?l / m L),followed by Bacillus subtilis,Bacillus cereus and rattan octopus.Turmeric volatile oil can significantly inhibit the growth of Salmonella,Bacillus subtilis,Bacillus cereus,and Garcinia cocci,especially after six hours,the growth of four test organisms was significantly inhibited.In addition,four test bacteria treated with volatile oil from turmeric.By measuring the conductivity of the four test bacteria and the leakage of the contents,it can be found that the conductivity of the four test bacteria treated with the volatile oil of turmeric was significantly higher than that of the untreated bacteria.The content has also risen.This shows that the volatile oil of turmeric can make the cells of the bacteria rupture,resulting in the leakage of the contents of the cells and eventually leading to the apoptosis of the cells.Fourth,the anti-inflammatory and analgesic activities of volatile oil from turmeric were studied.The turmeric volatile oil test was used to determine the turmeric volatile oil used in the experiment.The volatile oil concentration was 1.25 ?L/g in the low-dose group,2.5 ?L/g in the middle-dose group,and 4 ?L in the high-dose group 4?L/g.The anti-inflammatory activity of volatile oil of turmeric was detected by xylene-induced mouse ear swelling and carrageenan-induced mouse toe swelling.The experimental results showed that the volatile oil of turmeric in the middle and high dose groups could significantly inhibit the ear swelling of mice.Degree,and high-dose curcuma volatile oil also significantly inhibited the toe swelling of mice.By measuring the content of PGE2 in the mouse toe,it can also be seen that the PGE2 content in the inflammation toe of the turmeric-perfused turmeric group was significantly lower than that of the blank group.The analgesic activity of volatile oil of turmeric was detected by mouse hot plate experiment and mouse acetic acid-induced writhing test.The experimental results show that the volatile oil of turmeric in the middle and high dose groups can significantly reduce the number of writhing of mice caused by glacial acetic acid.And the high-dose group of turmeric volatile oil can significantly prolong the musculature response time.Finally,the in vitro anticancer activity of volatile oil of turmeric was studied.MTT assay was used to detect the inhibitory effects of volatile oil mouse melanoma cell B16,human lung cancer cell H1299,human hepatoma cell Hep G2 and human colon cancer cell line HCT116.The results showed that the volatile oil of turmeric has certain inhibitory effects on the four cancer cells.After treatment with 200 ppm,it is similar to the positive control group.Different treatment time,the inhibitory effect of each cell is not the same,24 h inhibition of Hep G2 best,the IC50 value of 91.4ppm 48 h inhibition of B16 is the most obvious,IC50 value of 44 ppm.It can also be seen by AO staining that the cell morphology of the cells treated with volatile oil of Turmeric has changed.Flow cytometry was used to analyze the apoptosis of curcuma volatile oil.The results showed that both H1299 and Hep G2 treated with volatile oil had increased numbers of early and late apoptotic cells,and the number of viable cells decreased,especially for Hep G2.The cell migration and colony formation assays were used to demonstrate the inhibitory effect of Curcuma Volatile oil on H1299 and B16 cells.The results showed that after treatment with 100 ppm curcuma volatile oil can significantly inhibit its migration.Especially for B16 cells,after treatment for 12 h,the blank group had healed,and the cells treated with volatile oil,the scratches not only did not close,but migrated outwards,and the intercell?Lar space increased,and the cells appeared shedding phenomenon.At the same time,colony formation was significantly inhibited in B16 cells treated with 50 ppm curcumin volatile oil.