Comparison On Detection Method And Study On The Ozonation Characteristics Of Ceftriaxone Sodium In Aqueous Solution

Antibiotics have made tremendous contributions to human life and health.They have been trusted by people.The abuse of antibiotics followed,and the abuse of antibiotics inevitably leads to environmental pollution.Antibiotics in the environment pose a threat to human health and ecological balance.Therefore,it is necessary to detect and remove antibiotics in aqueous solution.Ceftriaxone accounts for a large proportion in the production and use of antibiotics.Strengthening the detection and removal of ceftriaxone can control the contamination of antibiotics to some extent.At present,there is no uniform standard for the detection method of ceftriaxone sodium in aqueous solution.Different detection methods have different principles,have different features and precision,and use different instruments.The required instrument price and ease of operation vary greatly.The actual engineering needs to select the appropriate detection method according to different requirements.At present,there are no regulations on the discharge limits of antibiotics in domestic wastewater discharge indicators.Traditional biological and conventional physicochemical methods are not ideal for the treatment of antibiotics.Ozone oxidation is a practical and highly effective advanced oxidation technology.The reaction rate is fast,it is not easy to cause secondary pollution and no sludge is produced.It is a potential water treatment method.In this study,the chromatographic conditions for the determination of ceftriaxone sodium in the Pharmacopoeia by HPLC were modified and optimized.Three methods for the determination of ceftriaxone sodium in aqueous solution were compared experimentally:fading spectrophotometry,ultraviolet spectrophotometry,and high performance liquid chromatography,and analyzed;further study on the characteristics of ozone oxidation of ceftriaxone sodium:the operating conditions for ozone-oxidation of ceftriaxone sodium were optimized,and the reaction pathway and reaction products of ceftriaxone sodium were analyzed and determined.The results of the study are as follows:?1?The detection limits of high-purity water for the preparation of ceftriaxone sodium by fading spectrophotometry,ultraviolet spectrophotometry,and high performance liquid chromatography were 0.13,0.028,and 0.0047 mg·L^-1,respectively.The recovery rate reached 78⁸1%,92⁹6%and 99%¹01%,respectively.?2?When the ozone flow rate was 20.5 mg·min^-1,the optimal operating conditions for ozone-oxidation of ceftriaxone sodium were:influent concentration of 50 mg·L^-1,oxidation time 30 min,and ceftriaxone sodium removal rate of more than 90%.Ceftriaxone sodium removal rate can reach more than 90%,COD removal rate can reach about 30%.The oxidation process can be divided into two stages.In the first stage,ceftriaxone is converted into an intermediate product.In the second stage,the intermediate product is continuously oxidized,but it is difficult to be completely mineralized.?3?Ozone-oxidation of ceftriaxone sodium is greatly affected by pH,and ozone is directly oxidized under acidic conditions,and hydroxyl radicals?·OH?are indirectly oxidized under alkaline conditions.The two reaction pathways exist independently and can promote the decomposition of ceftriaxone sodium.In the first stage of the reaction,the direct oxidation of ozone is faster.?4?Addition of tert-butanol?TBA?under neutral conditions inhibits the indirect oxidation of free radicals and promotes direct oxidation of ozone.The oxidation rate is faster and the degradation effect is better.Addition of TBA under alkaline conditions inhibits both reaction pathways.The oxidation rate slowed down and the degree of oxidation decreased;adding hydrogen peroxide?H₂O₂?under neutral conditions can promote the ozonation of ceftriaxone sodium.