Sensors Based On Fenton Reaction With Gold Nanoclusters

Gold nanoclusters are a type of novel nanomaterials,with ultral-small size(<2nm)comparable to the Fermi wavelength of electons,they can provide extraordinary chemical and physical properties.Fluorescent metal nanoclusters have an attractive set of features,such as strong photoluminescence,excellent stability and good biocompatibility,making them ideal fluorescent probe to detect target substances.In this paper,we established a series of sensing systems based on the fluorescence quenching effect from hemoglobin and uric acid.In the first part of this work,gold nanoclusters were synthesized with bovine serum albumin(BSA)as the template material.As proved by the experiments,if this direct interaction scheme was to be applied for the quantification of Hb,only limited sensitivity could be obtained.In the case where Hb together with hydrogen peroxide was applied onto Au NCs,Fenton reaction could occur and form even more oxidative hydroxyl and hydroperoxyl radicals in the sensing system.Thus a sensor for sensitive detection of hemoglobin was developed.In the second part of this work,the fluorescence intensity of BSA-Au NCs in Fenton reaction for the concentration of hydrogen peroxide could sensitively respond,so the BSAAu NCs-Coumarin ratiometric fluorescent probes could greatly improve the detection of hydrogen peroxide by means of ferrous ions.As a product of a lot of physiological processes,the analysis of hydrogen peroxide could be extended to the test of uric acid.And the quantitative analysis of uric acid was achieved by detecting hydrogen peroxide.Due to high selectivity and excellent sensitivity,the sensor could be adopted in clinical and other applications.