Preparation And Application Of Silicon Particles And Metal Nanocluster Based Fluorescence Probe

Silicon nanoparticles?SiNPs?and metal nanoclusters?MNCs?have drawn great attention for their superior optical properties,good water solubility,simple preparation,low toxicity,excellect biocompatibility,and facile functionalization in the past decade.The merits of SiNPs and MNCs have been successfully used for sensing and bioimaging in the environment,biology,medical and other aspects.However,the sensor performance reported is not meet the requirement of trace level substances in vivo and in vitro detection.Therefore,it's very essential to improve the performance of SiNPs and MNCs probes and extend its versatility.In view of this,based on previous work,the dissertation carried out the following innovative work.1.The red fluorescent AuNCs were synthesized using BSA as a template and reducing agent,and AuNCs were used as fluorescent nanoprobes to establish a simple,rapid and sensitive detection method for Hg²⁺ in aqueous phase.It has be successfully applied to the detection Hg²⁺ in human serum and urine samples.2.For the first time,SiNPs/AuNCs fluorescent probes were prepared,and a new ultrasensitive and highly selective method for detecting Hg²⁺ in human serum and urine was established and applied to the live cell intracellular Hg²⁺ monitoring.Based on the competition mechanism between Hg²⁺-Cys and Hg²⁺-Au+,a new method for detecting L-cysteine was established.3.For the first time,SiNPs/CuNCs ratiometric fluorescent probes were prepared,and a highly sensitive fluorescence detection method was established,which has the potential for in vivo application.Fluorescence quenching was induced by the coordination between the amino and carboxyl groups of SiNPs/CuNCs and Hg²⁺.A new method for the determination of Hg²⁺ was established and monitoring Hg²⁺ in human serum and urine samples was achieved.