Design And Synthesis Of Fluorophor 1,8-Napthalimide Derivatives And Its Identification Of Cysteine

The fluorescence molecular probe detection technology has turned to be an attractive detection method owing to the advantages of easy operation,high selectivity,high sensitivity,low detection limit,minimal external interference,and nondestructive loroperty to the target product.Determination of sulfhydryl amino acid content is of great importance for disease research.Therefore,it is necessary to develop a fluorescent probe that can detect sulfhydryl amino acids.The 1,8-naphthalimide derivatives are fluorescein with brighter fluorescence and shorter wavelengths.Their application in fluorescent probes have been widely reported.In this paper,sixty one 1,8-naphthoyl derivatives were successfully synthesized by the nitration,oxidation and ammonolysis reactions.These compounds structure were characteriz--ed by IR,1H NMR,and were measured the performance by UV spectra and fluorescence spectra(FL).Unlike the non-fluorescent nitro-substituted compounds,the amino-substituted compounds had strong yellow fluorescence and the maleic anhydride-substituted compounds have weak blue fluorescence.Of the 19 probes,7 probes had a fluorescent OFF-ON effect on cysteine(Cys)solutions,it was found that these probes have the potential to detect cysteine.The specificity of the probe for cysteine detection was explored by adding 21 amino acids as distracters;The tolerance of the probe was studied by testing the fluorescence intensity at 8 different pH values;The Sensitivity of the probe was investigated by detecting the change of the fluorescence intensity of the probe and the cysteine line with the amino acid concentration.The response time of the probe was tested by fluorescence time spectral scanning;Through the above experiments,it was found that the probe showed a better sensitivity and selectivity for cysteine.The ability of seven fluorescent probes to be used for intracellular cysteine detection was explored by Hela cell fluorescence imaging and screened out probes with potential for biological detection.