Study On The Whole Algae Gel Product Development And Structure Identification Of Algin From Sargassum Fusiforme

Sargassum fusiforme is important economic seaweed.It belongs to the Sargassum family of the brown alga.And it is rich in nutrients and has high medicinal and edible value.However,the comprehensive utilization rate of Sargassum fusiforme is low.The purpose of this study is to make a whole algal gel product by using the gelatin properties of algin in Sargassum fusiform,and to isolate and purify the main component of Sargassum fusiform polysaccharide,algin,to study its structure and properties,which provides a theoretical basis for the comprehensive exploitation and utilization of the important economic seaweed Sargassum fusiforme.Firstly,based on the gel properties of algin in Sargassum fusiform,a whole algae gel product was developed and its main technology was optimized.The results show that the best way of preprocessing is ethanol soaking,the coagulant should choose two hydrated calcium phosphate.The process conditions were as follows:30%ethanol solution was used in the pre treatment stage and soaked Sargassum fusiforme at 40?for 40 min,and the ratio of the soaking solution was 1:20?g/m L?.Then at the stage of solidification,the ratio of beating is1:7?g/mL?,added 3%of two hydrated calcium hydrogen phosphatethe and gluconate lactone which the amount of substances such as calcium ions,and olidification time is 4 h.Next,after crude extraction by acid precipitation and protein removal by Sevag,align in Sargassum fusiforme was purified by DEAE-52 ion exchange column and Sephadex G-200gel column.And the structure of purified align was characterized by high efficiency gel filtration chromatography,infrared spectrum and nuclear magnetic resonance.The results showed that after 6 times of protein removal by Sevag,the protein in the crude align was basically removed;a homogeneous polysaccharide was identified by high performance gel filtration chromatography,and the relative molecular weight was 182 ku;algin absorption characteristic peaks such as 3434 cm^-1?-OH?,2919 cm^-1?C-H?,1617 cm^-11 and 1413 cm^-1?COO-?,1050 cm^-1?C-O of C-O-H?were identified by FTIR,so the algin in the polysaccharides of Sargassum fusiform was a uronic acid not containing sulphuric or protein,and containing two kinds of glycosidic bonds,?-and?-;and analysis of the fine structure of Sargassum fusiforme alginate by nuclear magnetic resonance spectroscopy showed that the ratio of?-D-mannuronic acid and?-L-guluronic acid was 0.98,and the average length of the G block was 16.2.Finally,the rheological properties of algin in Sargassum fusiforme polysaccharide were studied.The results showed that the sodium alginate solution from Sargassum fusiforme polysaccharide showed pseudoplastic fluid characteristics,but it was close to Newton fluid;at the same shear rate,the greater the concentration of alginate sodium solution,the greater the viscosity;the viscosity of sodium alginate solution decreased with the increase of temperature within the temperature range of 4⁸0?;within the pH range of 4¹0,the effect of pH on the viscosity of sodium alginate solution is not significant;the higher the concentration of the sodium alginate solution system change from stickiness to elasticity need the higher frequency;in the range of 0²5?,the solution system of sodium alginate is mainly sticky,and the solution system of sodium alginate is mainly elastic over 25?.In this study,the new whole algae gel product of Sargassum fusiforme was developed,and the structure of algin in Sargassum fusiforme was identified.It not only achieved the maximum utilization of Sargassum fusiform resources and improved its economic benefit,but also provided a theoretical basis for further development and utilization of Sargassum fusiforme.