| The electrochemiluminescence sensor is a type of sensor based on electrochemiluminescence technology.The electrochemiluminescence technology has the advantages of high sensitivity,fast analysis speed,low signal background and strong controllability,and has been widely used in recent years.The main development direction of electrochemiluminescence technology is the development of luminescent materials.As an emerging carbon nanomaterial,carbon quantum dots have unique optical and electrical properties,and their advantages of ease of synthesis,high efficiency,non-toxicity,and good biocompatibility make them a good foundation for the construction of environment-friendly electrochemiluminescence sensors.This thesis aims to construct electrochemiluminescence sensors based on carbon quantum dots.In general,the design idea of electrochemiluminescence sensors is different for different analytes.Commonly,the detected analytes are proteins,nucleic acids,biological molecules,and metal ions.These analytes are closely related to human health and living environment.Adenosine triphosphate(ATP)plays an important role in cell metabolism as the main energy source in the human small molecule,it's concentration and consumption rate can be used as indicators of various diseases such as hypoxia,hypoglycemia,Parkinson's disease and certain malignant tumors.Similarly,Cu2+ is one of the metal ions in water which is usually from the metallurgy,chemical,electroplating and other industries,and due to its large mobility,wide application,and difficult degradation,it can be enriched in the body through various forms.If Cu2+ is at a high concentration for a long time in the body,it will lead to metabolic disorders,hypertension,coronary heart disease and other diseases.Therefore,this dissertation aims to construct electrochemiluminescence sensors based on carbon quantum dots for the detection of ATP and Cu2+,and realize the detection of actual samples.The development of such sensors is of great significance for human health and ecosystem stability.The specific work is as follows:1?NCQDs were synthesized by pyrolysis of diethylenetriaminepentaacetic acid,and their morphology,optical and electrical properties were characterized.K2S2O8 was used as a cathode common reaction reagent.After optimization of the mass per unit area loading of the electrode surface,the concentration of the co-reactants,the PBS concentration of the test liquid,and the scanning rate of the NCQDs,the excellent electrochemiluminescence performance was measured.Electrochemiluminescence sensing analysis.ATP was used as a detection model,NCQDs were used as electrochemiluminescence materials,and ATP-aptamer was used as a material that specifically recognizes ATP.Modified probes GCE/NCQDs/ATP-aptamer were prepared after the best preparation conditions were explored.After modification conditions,modified electrodes GCE/NCQDs/ATP-aptamer were prepared for the detection of ATP.The detection limit was as low as 0.43 pmol/L(signal to noise ratio was 3),lower than the reported electrochemiluminescence method.At the same time,the sensor has good stability and anti-interference,and can be applied to the actual serum samples,the actual sample analysis found that the sensor has good accuracy and feasibility.2?We choose the NCQDs prepared by the above pyrolysis method as electrochemiluminescence materials,by using chitosan as a binding agent and adsorbing agent,the mixture of the two was used to prepare modified electrodes GCE/NCQDs/Chit by optimizing the optimal preparation conditions.Using the electrochemiluminescence-energy resonance transfer principle,the electrochemiluminescence of NCQDs can be specifically quenched by Cu2+,enabling the detection of Cu2+ in water,exhibiting a wide linear range(1.1 to 310.4 nmol/L),and low The detection limit(0.25 pmol/L)and electrochemiluminescence sensor also have good selectivity and stability,which can be used to detect Cu2+ in real samples. |
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