Study On Novel Solid-state Electrochemiluminescence Sensors And Its Applications In Bioanalysis

Electrochemiluminescence (ECL) is a technology that perfectly combineselectrochemistry and chemiluminescence. As ECL has the characteristics of highsensitivity, wide linear rage, quick detection process and good controllability, it is nowoccupying an important place in the analytical chemistry research and has been widelyapplied to almost every fields of analysis. Among all the traditional ECL system,Ru(bpy)32+and its derivatives are the most widely used due to its outstandingcharacteristics, such as good water solubility, high ECL efficiency, good chemicalstability and electrochemical renewability. Solid-state ECL sensors based on theimmobilization of Ru(bpy)32+is a hot topic because it has the advantages of savingreagents, high ECL intensity and reusability. Quantum dot (QD) is a newly developedbranch of ECL system in recent years and rapidly established its important position inthe ECL field due to its unique optical、electrochemical and ECL properties.Based on the discussions above, we mainly focused on the following three works.1. Based on the electrostatic interaction between Ru(bpy)32+and cancer cells, wesuccessfully captured cancer cells on the pentenyl functionalized Ru(bpy)32+immobilized indium tin oxide (ITO), developing a simple, rapid and label-free ECLprotocol for leukemia cell detection. The detection method was successfully appliedfor studying the growth of Jurkat cells and evaluating the cytotoxicity of chemicalsusing cycloheximide as a model, indicating its potential applications for screening ofanticancer drugs.2. Based on the alkenyl radical polymerization reaction, we immobilized pentenylfunctionalized Ru(bpy)32+onto the vinyl triethoxy silane (TPS) treated ITO in thepresence of initiator, developing a novel ECL sensor. The sensor has the characteristicsof easy preparation, high sensitivity, good chemical stability and satisfactory stabilityin organic solvent. The sensor also exhibited excellent behavior to tripropylamine(TPrA), indicating the potential application for tertiary amines detection.3. By the way of immobilizing the synthesized poly(diallyldimethylammoniumchloride)(PDDA)-protected graphene-CdS (P-GR-CdS) composites composite ontogold electrode, we developed a novel ECL sensor. The sensor exhibited satisfactoryECL efficiency, excellent electrochemical stability and can be reused. It also showedan excellent ECL behavior to glutathione (GSH), providing a sensitive protocol for GSH detection.