Study On The Preparation Of Three-dimensional Scaffold Materials By Macroscopic Supramolecular Assembly

Tissue engineering is a revolutionary technology for constructing bioactive tissues in vitro to repair or replace damaged organs.As an important part of tissue engineering,three-dimensional(3D)scaffold material provides mechanical support and a good microenvironment for cell growth.Currently,how to simulate the 3D growth environment in vivo and preparate 3D scaffolds materials with chemical and biological has become an important challenge in this field.In recent years,the newly developed macroscopic supramolecular assembly has developed into a new principle and new method for the preparation of 3D scaffold materials with advantages of mild experimental conditions,relatively simple operation and strong material adaptability and so on.This thesis tried to address the problems of poor biocompatibility and selective adhesion of macroscopic assembly,mainly using biocompatible natural biomolecules to construct a compatible 3D scaffold structure by macroscopic supramolecular assembly;the selective adsorption and growth of cells in 3D space were realized by natural selection mechanism.The main results contributions are as follows:1?Biocompatible polyelectrolyte multilayers were designed and prepared,and 3D scaffolds were fabricated by external magnetic field assisted macroscopic supramolecular assembly.The results of cell adsorption experiments show that the as-prepared 3D scaffold displayed the excellent spreading and the high proliferation rate in vitro,realizing the construction of 3D scaffold with cell compatibility.2?With the premise of ensuring biocompatibility,a 3D scaffold containing poly-L-lysin is successfully prepared on the substrate containing poly-D-lysin through macroscopic supramolecular assembly,using the difference in the adsorption of chiral to cells and proteins in nature.The results of cell experiments showed that the supporting substrate leads to fibroblasts selectively adsorb and excellently grow on the prepared 3D scaffolds,achieving the 3D environment of selective adsorption and growth of the cells.Therefore,macroscopic supramolecular assembly fully embodies the advantages of the preparation of chemically specific 3D scaffolds,and is expected to provide a possibility for the preparation of complex 3D microenvironment to simulate the growth of cells in vivo,thus laying the foundation for the reconstruction of tissue in vitro.