The Synthesis And Biological Application Of Organic Fluorescent Dye BODIPY

The boron-dipyrrole-methene dye(BODIPY,BDP),as one of the novel organic fluorescent dyes,has aroused people's wide concern and become one hot research fields,due to their excellent stability to light and chemicals,high fluorescence quantum yield.In this paper,we focused on combining BDP with chemotherapy drugs for tracing drugs as well as the heavy atom being introduced to enhance photodynamic therapy effect.1.A new antitumor prodrug(BDP-SS-CPT),which combined by fluorescent dye(BDP),the antitumor drug(CPT)and a disulfide linker,was designed and synthesized.Simutaneously,BDP-SS-CPT can self-assemble into nanoparticles.When BDP-SS-CPT was exposed to GSH,the disulfide linker will be broken with release of BDP and CPT.The absorbance spectra have changed apparently and the fluorescence emission spectra have prominent enhancement.The fluorescence ratio of BDP and CPT as well as confocal fluorescence imaging could be used to tracing the release of CPT.Meanwhile,the released CPT could be used for chemotherapy.2.A new fluorescent cyclometalated Ir(III)complexes(IrBDP)containing BDP was designed and synthesized.IrBDP could self-assemble into nanoparticles(Ir BDP NPs).The introduction of Ir(III)significantly increased the photodynamic effect of BDP.Ir BDP NPs have excellent photo cycotocxicity against cancer cells.3.A new near-infrared(NIR)multifunctional BODIPY-Platinum(BDP-Pt)conjugate was synthesized,which composed of fluorescent dyes(BDP)and therapeutic agents(cisplatin).BDP-Pt conjugate combines NIR imaging,photodynamic therapy(PDT)and chemotherapy.BDP-Pt possesses the excellent phototoxicity at low concentration under irradiation and comparable chemotherapeutic toxicity with cisplatin.Simultaneously,BDP-Pt maitains the NIR fluorescent imaging and antitumor effect to tumor-bearing mice.