SERS Spectral Detection Of Microalbuminuria And Folic Acid By Immune-regulated Nanocatalysis Of Gold And Silver Nanoparticles

Part ? IntroductionIn this paper,the progress of surface-enhanced Raman scattering spectroscopy,the application of SERS substrate and SERS spectroscopy in immunoassay were introduced.The qualitative analysis of aptamers,SERS analysis of enzymes and the application of carbon nanomaterials in SERS analysis were also introduced.Meanwhile,catalytic,antigen-antibody-specific reactions such as graphene oxide nanoribbons and carbon quantum dots and SERS and RRS spectra are reviewed.With the combination of technology,immunoregulatory SERS spectroscopy was established to detect microalbumin and folic acid.Part ? Immunoregulation of GONR catalytic reaction-SERS detection of m AlbHighly catalytic graphene oxide nanoribbons?GONR?were prepared by KMnO₄ oxidation of multi-walled carbon nanotubes?MWCNTs?.The reaction of HAuCl₄-H₂O₂,which was slow under the condition of 60? water bath,was catalyzed strongly by GONR to generate gold nanoparticles?AuNPs?that exhibited a strong surface-enhanced Raman scattering?SERS?peak at 1615 cm^-1 in the presence of molecular probe VBB,and the SERS intensity enhanced with GONR concentration increase due to the generated AuNPs substrate increasing.Microalbuminuria antibody?Ab?bound with GONR to form Ab-GONR that inhibited the catalysis.When the urine microalbumin?mAlb?was added,the specific immunoreaction took place to form stable Ab-mAlb immunocomplex which was released from the GONR surface,and the SERS signals enhanced due to the restore of nanocatalytic effect of GONR.The enhanced SERS signals were linear with the m Alb concentration in the range of 0.065-2.62 ng/m L,with a detection limit of 0.02 ng/m L.So,a new SERS quantitative analysis method for m Alb was established based on the immunoregulation of GONR catalysis and the gold nanoplasmon.Part ? Immune-regulated carbon spot catalytic Ag NO₃-Glu reaction-SERS detection of folic acidIn the 60? water bath,the reaction of GLU-AgNO₃ system is slowly,and the addition of carbon dot?CD?nanozyme catalyst synthesized at high temperature from citric acid and urea accelerates the reaction and increases the number of silver nanoparticles produced in the system.When add the Victoria blue B?VBB?molecular probes,the system showed a strong SERS effect.Folate antibody?Ab?can bind to CD to form Ab-CD and inhibit its catalytic action.In the presence of folic acid?FA?,it specifically immunoreacts with Ab,and the Ab-FA immune complex exits the CD surface,activating the CD nanocatalytic effect.As the FA concentration increases,the system SERS signal increases linearly.When the FA concentration was in the range of 0.1-2ng/ml,the ?I_1615cm-1 value showed a good linear relationship with the m Alb concentration.The detection limit was 0.048ng/ml and the correlation coefficient was 0.9826.Based on this,a novel method for the quantitative analysis of immunoregulatory CD-catalyzed reaction-SERS for the detection of FA was established.The results were satisfactory.