GO/GONR Catalysis-SERS And Fluorescence Determination Of Trace Ferritin And Biotin,respectively

Part ? IntroductionThe progress of surface-enhanced Raman spectroscopy,SERS enhancement mechanism,analysis and application of SERS spectroscopy,aptamer SERS analysis,enzyme SERS analysis of molecular fluorescence spectroscopy and its application in environmental analysis are introduced.The recent advances in the research of nano-enzymes,major nano-enzymes,their preparation methods and nano-enzyme catalysis were reviewed.A brief overview of aptamer analysis techniques and their analysis and application in different fields is provided.The analysis and research progress of ferritin and biotin were introduced.The main contents and significance of this research were briefly described.Part ? SERS quantitative analysis of trace ferritin based on immunoreaction regulation of graphene oxide catalytic nanogold reactionIn 50? conditions,the gold nanoreaction of HAu Cl4-H2O2 is very slow,graphene oxide(GO)exhibitedb strong catalysis on the nanoreaction to form gold nanoparticles(Au NPs)with strong surface-enhanced.Raman scattering(SERS)activity at 1614 cm-1 in the presence of molecular probes of Victoria Blue(VB4r).The SERS intensity increased with the GO increasing due to formation of more Au NPs.The ferritin antibody(Ab)can bind to GO surface to form Ab-GO that can inhibit the nanocatalysis.When ferritin(Ag)existed,the produced immunocomplexes of Ab-Ag are separated from the GO surface,which led to the recovery of GO catalysis.The enhanced SERS signal I1614cm-1 is linear to ferritin concentration in the range of 0.017–1.02 ng/m L.Accordingly,a new SERS quantitative analysis method was established for detection of ferritin,based on the regulating GO nanocatalysis.Part ? Affinity Regulated GONR Catalyzes Na H2PO2-RH6 G Reaction-Fluorescence/Abs/SERS Assay for BiotinIn the presence of 7.5 mmo L/L Na AC-HCl at 80?,the reaction of Na H2PO2-RH6 G system proceeds slowly.The addition of graphene nanoribbons(GONR)nanozymes as catalysts makes Rhodamine The 6G fluorescence intensity is significantly reduced.When avidin and biotin are combined,the fluorescence reaction can be suppressed and fluorescence brightness can be reduced.Based on this,a fluorescent method for indirect detection of biotin can be established.As the concentration of the analyte increases,the fluorescence signal of the system gradually decreases.Biotin concentration in the range of 0.0375-0.5ng/m L was in good linear relationship with the system MFS signal reduction value ? F550 nm.The linear equation was ?F550nm=1099.2C+5.6986.The correlation coefficient was 0.9859.The detection limit was 0.015ng/m L.Based on this,a new method for quantitative analysis of fluorescence spectra for the determination of biotin can be established.