Synthesis And Application Of Rhodamine Fluorescent Probes Targeting Labeled Proteins

Fluorescent dyes are widely used in the biological field and become an indispensable tool in the field of life science.With the advancement of science and technology,it has become a hot spot for scientists to target-trace the living tissues in order to accurately analyze the properties and characteristics of each structure.As the basic structural unit of eukaryotic organisms,cells have extremely important existence value in the process of life.Therefore,it is practical and important significance to explore the intracellular environment and cell composition structure for the analysis of physiological processes and disease treatment.The nucleus is the largest structure of intracellular genetic material storage,and control important processes such as cell division,growth,metabolism,and aging,and has become a hot research in the medical and biological fields.The protein that constitutes the material basis of cells is a key structure that regulates the growth and metabolism of cells.At present,the most widely used method for protein research is the labeling method of protein-targeting fluorescent probes.The principle is to design fluorescent probes with good water solubility and light stability through chemical synthesis,linking targeted protein labels,through fluorescence Microscopic imaging cell experiments accurately localize proteins on subcellular organelles.Currently,a wide variety of fluorescent dyes have been researched and developed.Among them,rhodamine is widely used in cell imaging experiments due to its excellent optical properties and good water solubility.In the second chapter,Rhodamine designed three fluorescent probes targeting the cell nucleus to track and explore the nucleus.This kind of fluorescent probe was synthesized and the asymmetric route was selected.Functional groups with excellent optical properties were added on the derivatization side of Rhodanmine to increase the luminescent properties and water solubility of the probes.These three fluorescent probes were applied to cell imaging experiments and analyzed by fluorescence microscopy to verify that they could be stably labeled on nuclear proteins.And through super-resolution imaging experiments,can clearly observe the target molecular marker nucleur outline and structure.In chapter 3 of this article,a fluorescent probe with a pKa near-neutral targeting protein tag was synthesized.We know that most of the subcellular organelles in the cell are close to neutral in pH,and very few types of fluorescent probes of this near-neutral pKa have been reported.Therefore,a symmetrical rhodamine probe was designed in this study.The pKa regulation was achieved by intramolecular proton transfer,and the target protein tag was attached to achieve the targeted localization of neutral subcellular organelles and the detection of pH.The cell fluorescence microscopic imaging technique verifies that the probe can be stably labeled in the nucleur of the neutral pH environment,and through the vitro test,this probe pKa value is 6.The near-neutral pKa has important application value in the detection of multiple subcellular organelles in the cell.And the probe molecules can be used in the super-resolution imaging,and can be specifically and stably located in the microtubule,could be used in the field of targeted labeling and biological applications.