Preparation Of Poly (N-isopropylacrylamide-co-3-acrylamidobenzeneboronic Acid) Glucose Responsive Gel Fibers By Microfluidic Method

In recent years,the incidence of diabetes is climbing steadily and tends to be younger in our country.Diabetes can cause serious complications such as heart disease,retinal detachment and kidney disease,which seriously endanger human health.Monitoring and controlling blood glucose concentrations are very important in the treatment of diabetes.Traditional acupuncture blood to measure blood glucose are not only painful and ineffective for patients,therefore,the development of glucose-responsive hydrogel fibers that are capable of long-term monitoring of different concentrations of glucose solutions has gradually become a research hotspot.The sensitive hydrogel with phenylboronic acid as the sensitive element is an all-synthesized system,which has the advantages of good stability,low toxicity,no immune rejection,low production cost,et al,and it can be widely used in blood glucose monitoring.For the past few years,the developed microfluidic technology for the preparation of glucose gel fiber provides a new way.In order to explore the synthesis conditions of glucose-sensitive gel fibers prepared by microfluidic spinning method,glucose-responsive hydrogel key functional monomers 3-acrylamidobenzene boronic acid(AAPBA)were first synthesized in this paper.Then the poly(N-isopropylacrylamide-co-3-acrylamidophenylboronic acid)(P(NIPAM-co-AAPBA))glucose responsive hydrogels were prepared by free radical polymerization,the effects of the monomer ratio on the hydrogels response behavior were investigated.Finally,fast response P(NIPAM-co-AAPBA)glucose responsive gel fibers were synthesized by self-made T-type microfluidic chip based on microfluidic instant spinning technology coupled with photo-initiated free radical polymerization.The main research results are as follows:1.In alkaline solution,3-aminophenylboronic acid and acryloyl chloride were used as raw materials to synthesize 3-acrylamidophenylboronic acid(AAPBA)in ice-water bath.The product was purified to get a white solid powder.The product purified was verified by elemental analysis,Fourier transform infrared spectroscopy,1HNMR and MS.2.P(NIPAM-co-AAPBA)glucose-responsive hydrogel was synthesized by free radical polymerization by using chemical cross-linking agent N,N'-methylenebisacrylamide(BIS)or physical cross-linking inorganic(Clay).The effects of different cross-linking agent and monomer ratio on the structure of the gel and glucose response were discussed.The results show that Clay can replace traditional chemicalcross-linking agents to prepare hydrogels.Compared with traditional hydrogels,Clay prepared hydrogels have excellent mechanical properties.The monomer ratio has a significant influence on the structure,swelling kinetics and glucose responsiveness of P(NIPAM-co-AAPBA)hydrogel.With the mass increase of AAPBA in the gel network structure,the pore structure of the gel,the swelling rate and equilibrium swelling decreased.The gel mass significantly increased in the glucose responsiveness test.3.Fast response P(NIPAM-co-AAPBA)glucose responsive gel fibers were synthesized with uniform structure by self-made T-type microfluidic chip based on microfluidic instant spinning technology and photo-initiated free radical polymerization.The studies have shown that the size of the gel fibers can be effectively controlled by adjusting the flow rate of the core and the shell.Characterization of gel fibers by Fourier transform infrared spectroscopy and EDS-mapping confirmed that NIPAM and AAPBA successfully formed gel fibers.The swelling kinetic,glucose responsiveness and mechanical properties of the gel fibers were investigated.The results showed that the gelatin fibers exhibited rapid glucose responsiveness.The gel fiber prepared by Clay could improve the mechanical properties remarkably.