Study On The Preparation And Characteristics Of Representative Components In Anthocyanin Extract

Due to the low instability and content of anthocyanins,it is difficult to obtain a large number of high-purity anthocyanins by traditional methods for separating anthocyanins.High-speed countercurrent chromatography HSCCC is more efficient,stable,and has a large amount of preparation.It is used for the separation of natural active ingredients.The establishment of an efficient preparation technique for an anthocyanin representative monomer compound is of great significance for further study of the mechanism of action and product quality control.In this experiment,purple sweet potato,black wolfberry,mulberry and blackcurrant anthocyanin extract prepared starting from its monomeric anthocyanin components and antioxidant properties in vitro studies.Selecting each of the anthocyanins to have a large content,a middle molecular weight and a representative monomer compound as the target components,the high-speed countercurrent chromatography was used to prepare and separatefour sources of anthocyanins.Methyl tert-butyl ether-n-butanol-acetonitrile-water-trifluoroacetic acid was used as a solvent system.The K value of different solvent systems was measured by HPLC,the optimal solvent system ratio was selected,and the flow rate was set to The high-purity anthocyanin monomer compound was isolated at 5 mL/min at a rotation speed of 850 r/min.The final choice of the solvent system ratio was methyl tert-butyl ether-n-butanol-acetonitrile-water-trifluoroaceticacid=2:5:1:6:0.01.Preparation and separation of four purple sweet potato glucoside monomer components and selection of solvent The ratio of the system was methyl tert-butyl ether-n-butanol-acetonitrile-water-trifluoroacetic acid=2:2:1:5:0.01.Preparation A black anthocyanin monomer component and 4 black gallon colors were obtained.The glycoside monomer component and the two kinds of mulberry anthocyanin monomer components were each selected to have a large content of each anthocyanin and a middle molecular weight,and a representative monomer compound was selected as a representative component.In this experiment,semi-preparative high performance liquid chromatography was used to separate and prepare the purple sweet potato anthocyanin monomer components,and purple sweet potato anthocyanin monomer compounds with higher purity were obtained.In this experiment,the content and composition of anthocyanins were determine d by spectrophotometry and HPLC-MS.The antioxidant activity in vitro was analyze d by DPPH radical,hydroxyl radical scavenging capacity and total reducing power.The conditions of light and temperature were studied.The effect of the stability of the monomer compounds from different sources.The results show that the representat ive components of anthocyanin in the four sources were Peonidin-caffeoyl-feruloylso phoroside-5-glucoside,petunidin-3-6-p-cournaryl-rutinoside-5-glucoside,Cyanidin-3-rutinos ide and Cyanidin-3-glucoside,All of them have good in vitro antioxidant activity,an d there are differences in stability.Purple sweet potato anthocyanins have good stabi lity.The purity of the four representative monomer compounds was 90.51%,94.25%,95.02%,and 98.2%,the molecular weight of the four representative monomer com pounds m/z was 1125,933,595 and 449 respectively,as determined by high performan ce liquid chromatography and MS.