Preparation And Properties Of Multicomponent Electrospun Membranes Containing Natural Antibacterial Agent Eugenol

Confronted with the ubiquitous risks of thrombosis,stenosis and bacterial infection,vascular materials are necessarily required with rapid endothelialization,anti-nonspecific adhesion and antibacterial activity.In this work,eugenol,a plant-extracted natural antibacterial agent,with broad-spectrum antibacterial activity and low biotoxicity was introduced into the materials.Meanwhile,the short active peptide REDV and the zwitterionic polymer poly?sulfobetaine methylacrylate??PSBMA?were added in the materials,respectively,to obtain antimicrobial poly??-caprolactone??PCL?/gelatin electrospun membranes and polyurethane?PU?composite films endowed with endothelialization and anti-adhesion,respectively.REDV was covalently conjugated with a low molecular weight PCL to obtain REDV-PCL-REDV,which was then electrospun with gelatin and high molecular weight PCL.The prepared electrospun membranes could be decorated by REDV,and eugenol was encapsulated into the electrospun fibers to realize controlled and sustained release.The electrospun membranes presented acceptable mechanical properties and release profiles.The electrospun membrane containing 30%of eugenol could efficiently inhibit Escherichia coli?E.coli?and Staphylococcus aureus?S.aureus?growth with 71.6�3.3%and 78.6�2.5%of inhibition rates,respectively.Besides,the electrospun membranes exhibited lower cytotoxicity towards L929 fibroblasts with more than 80%of relative cell viability,and the REDV-modified membranes could evidently facilitate VEC adhesion and proliferation.Eugenol was covalently conjugated to chitosan backbone to synthesize the chitosan-g-eugenol?CE?and the antifouling copolymer poly?sulfobetaine methylacrylate-co-2-aminoethyl methacrylate??PSA?was synthesized via reversible addition-fragmentation chain transfer polymerization.Then,CE was cross-linked with PSA on the surface of PU electrospun membrane taking genipin as a cross-linking agent to obtain composite film PU/CE/PSA.The PU/CE/PSA film not only presented excellent antibacterial activity with 92.8�2.5%and 95.2�1.3%of growth inhibition rates against E.coli and S.aureus,respectively,but also could significantly resist nonspecific protein adsorption,platelet and bacterial adhesion with adsorption amount of 1.1�0.2?g/cm² for bovine serum albumin,1.9�0.1?g/cm² for bovine fibrinogen,and adhesion number of?3.1�0.4?�10⁴ cm^-2 for platelets.Meanwhile,the cross-linked composite film exhibited low hemolytic activity with hemolysis ratio<2%and cytotoxicity towards L929 fibroblasts with relative cell viability>80%.