| In recent years,the global cancer cases showing rapid growth.The World Cancer Reportforecasts that cancer incidence and mortality rates will continue to rise in our country in the next few years.Therefore,prevention and treatment of cancer has attracted widespread attention.Currently,cancer treatment method mainly to drug-based.So far,platinum anti-cancer drugs is the most effective anti-cancer metal drugs in clinical application.Three platinum-containing drugs are approved worldwide for treating cancer in humans,cisplatin,carboplatin,and oxaliplatin.But platinum anticancer drugs have greater side effects and drug resistance,greatly limits its further application.Therefore,look for other low toxicity and resistance anticancer metal complexes have become the anti-tumor therapy research focus areas.As we all know,carboxylic acids metal complexes have a diverse and stable structure,play an important role in the coordination chemistry.In recent times,there have remained intensely about focusing on the design of new metal based anticancer drugs that display improved selectivity and novel modes of DNA interaction similar to non-covalent interactions that mimic the mode of interaction of biomolecules.On the other hand,Mn(II),Zn(II),Cu(II)ions play critical roles in dioxygen metabolism of many biological systems and corresponding to their complexes can affect the translation and transcription of DNA double strands.Mn(II)complexes show anticancer /antiproliferative activities.Zn(II)complexes are utilized for the treatment of Alzheimer disease,and can act as radio protective agents,tumor photosensitizers,anticonvulsants,antidiabetic insulin-mimetics,anti-inflammatories and show cytotoxicity against human cancer cell lines.Some Zn(II)complexes can strongly bind and cleave DNA,they exhibit prominent anticancer activities and regulate apoptosis ability.The kinetically labile Cu(II)ion has a thermodynamic driving force for DNA complexes.All in all,such complexes play an important role in the endogenous oxidative DNA damage associated with aging and cancer,but it has been proposed that endogenous metals like copper may be equally effective but less toxic than platinum complexes.Based on the above facts,we selected transition metal ions(Mn(II),Zn(II),Cu(II))to synthesize the novel complexes.Although many metal complexes with anticancer activity and apoptosis induction have now been synthesized,few studies have been conducted on how to deliver such a complex to a target site.Therefore,we have chosen an inorganic material—magnesia,to support such complexes.This paper mainly includes the following several aspects:1? Fore single crystal metal complexes were prepared under hydrothermal conditions and the structures of these complexes were determined by single crystal X-ray diffraction and characterized by IR analysis and elemental analysis.2? The micro-nano-scale magnesia was synthesized.The morphology,particle size and dispersion of magnesia were characterized by SEM,XRD and particle size distribution analyzer.Fourier transform infrared spectroscopy(FT-IR)were used to analysis the information of the functional group.3? Using MgO to supported metal complex 4,and measuring the drug loading and entrapment efficiency.The loaded product was characterized by SEM.4? Fluorescence spectroscopy and gel electrophoresis were used to study the interaction between the complexes and DNA.The results showed that the complexes mainly interacted with DNA by insertion.5? Cytotoxicity of the complexes to cells were studied by flow cytometry and fluorescence inverted microscopy.The experimental results showed that the ability of complex 4 to induce apoptosis is the strongest of these four complexes.6? The anticancer activity of the loaded product and the ability of inducing apoptosis were characterized by gel electrophoresis,flow cytometry and fluorescence inverted microscopy. |
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