Study On The Analysis Methods Of Peanut Allergens In Food

Food allergy represents an important food safety issue because of the potential lethal effects.The only effective treatment is to avoid eating allergen foods.However,during the process of manufacture,machining,storing,transporting and selling,foods may be unintentionally contaminated by food allergens.Therefore,it is of great significance to quantify the amount of food allergens in a food for preventing the anaphylaxis.This paper aims to set up a testing platform of the plant allergy based on liquid chromatography-mass spectrometry and Proteomics and carry out a reseach about the residue of allergens in food.In this paper,peanut was Chosen as the main research object.A reliable ultra-high-performance liquid chromatography-mass spectrometry method for determination of peanut allergen was developed based on protein databank and specific peptides.Peanut allergen Ara h 1and Ara h 2 was quantified by employing the synthetic internal standard based on the molarequivalent relationship among the internal standard,Ara h 1?Ara h 2 and their signature peptides.A reliable ultra-high-performance liquid chromatography-mass spectrometry method for determination of peanut allergen Ara h 1and Ara h 2 was developed.Compared to the previous methods,the developed approach with mass spectrometry operated in multiple reaction monitoring mode offered a highly specific,sensitive and accurate method.The established method was extensively validated by determining sensitivity(limit of quantitation ?g/g and 4.45 ?g/g)and recovery(96.6%?100.0% and 106.0%?107.8%).Current validated method was successfully applied to the peanut allergen Ara h 1 and Ara h 2 content in 20 kinds of peanut from Chinese different regions.And the results showed the conversion coefficient of peanut allergen Ara h 2 and peanut protein.Thus,Ara h 2 was chosen as biomarkers to detect peanut protein in 10 kinds of roasted food.And the established method was extensively validated by determining sensitivity(limit of quantitation 6.23 ug/g)and recovery(107.0%?113.2%).