Study On Separation Of Multi-layer Flexible Free-standing Molecularly Imprinted Membrane For Genistein In Radix Puerariae

Natural medicine in traditional Chinese medicine is complex,which contains the active ingredient of purification is an arduous and painstaking work,and it is also the emphases and difficulties of restricting internationalization of traditional Chinese medicine.Genistein of active ingredients in radix puerariae due to some similar structural component to its existence and content is very low,so how effective extraction of genistein from kudzu as a hotspot.Now we couple with membrane separation technologies and Molecular Imprinting technique,prepare a new multi-layer pore flexible self-supporting molecular imprinted membrane(MIM)with specificity identify and isolate to genistein,accomplishing the separation and purification of genistein in Pueraria.1.A multi-objective optimization technique based on response surface meth-od was used to optimize the processes of ultrasonic-assisted extraction of five different isoflavonoids from Radix Puerariae,the optimal extraction conditions:ethanol concentration72.5%,extraction temperature58(?),extraction time 49(min),ultrasonic power 360(W).By confirmatory experiments to test the relia bility of the model,the results are compared with the predictions of the softw are,and fitting were:102.28%,99.81%,101.61%,99.10%,102.59%,indicate the e-xtraction rate of consistency with the model equations corresponding forecast v alues are good.2.Prepared of SiO2 microspheres with sol-gel method,and discussed the effects of the main factors on preparation process the amount of ammonia,the amount of ethanol,the amount of water,the amount of TEOS,and the reaction temperature,The SiO2 Microsphere particle with this method size uniformity,spherical high,good dispersion.3.Multi-layer flexible free-standing molecularly imprinted membrane was pr-epared genistein as template molecular,methyl acrylic(MAA)and 1-Vinylimid azole(VMZ)as function monomer,[Bmin]Br ion liquid and ethanol as solvent,SiO2 microspheres as macroporous pore-forming agent,EDGMA as crosslinkin g agent,ABIN as initiator.Study the influences of MIM with different particle size of SiO2 microspheres and differentmass fraction of SiO2 ethanol solution on the adsorption capacity.Explored the adsorption capacity of MIM with thre-e different particle sizes by adsorption kinetics,and using first order Kinetic and second order kinetics curves fitting to study the adsorption mechanism of MIM;Explored the adsorption properties of MIM for genistein by static adsor ption,researched and analyzed the combination characteristics of MIM for geni-stein,with Langmuir,Freundlich curve fitting methods for MIM on adsorption mechanism of genistein.Through penetration testing found that genistein in MI-M penetration mass transfer mechanism of priority in.Finally,MIM and solid-phase extraction combined use of genistein flavone in pueraria lobata extracts the isolation and purification,the results show that MIM on the separation and purification of genistein has a very good effect.