Applications Of Phosphorescent Sensors For Active Pharmaceutical Ingredient With The Mn-doped ZnS Quantum Dots As The Phosphorescence Probe

In this dissertation,the research strategies of phosphorescence probe for selective and sensitive determination of designated active pharmaceutical ingredient is proposed with the Mn-doped ZnS quantum dots as a phosphorescent sensor.It consists of the following four chapters.In the preface,the essential properties,preparation and characteristic method of QDs and QDs-doped are described.The applications of Mn:ZnS QDs as a kind of phosphorescence probe are summarized too.Then,the investigative targets,significance and processes in this article are proposed simultaneously.In chapter 2,a new phosphorescence probe for the selective detection of metronidazole(MTZ)was developed based on the phosphor-escence quenching of L-cysteine-capped Mn-doped ZnS quantum dots by MTZ.Experimental results show that a good linear relationship between the phosphorescence intensity quenching(APL)and the concentration of MTZ can be obtained in the aqueous media of Tris-HCl buffer solution(pH=7.50).The calibration equation are APL = 19.75+1.45c(MTZ)and ?PL=186.2+0.29c(MTZ)in the two concentration range of MTZ respectively.The method has been used to determine MTZ in MTZ tablets with 97.92%and 100.82%recoveries.In chapter 3,the glutathione-capped Mn-doped ZnS quantum dots(Mn:ZnS-GSH QDs)as a phosphorescent probe for determination of dopamine(DA)is proposed.The results show that the concentration of good linear relationship between the APL and DA in 0.10?3.0 ?qmol/L of concentration range can be obtained in the Tris-HCl buffer solution(pH=8.60).The calibration equation is ? PL = 15.30 + 84.90c(DA)with a correlation coefficient(r)0.9965 and a linear range of 0.08?3.0 ?mol/L.The method has been applied to determine DA in a dopamine hydrochloride injection with 96.50%recovery.In chapter 4,a phosphorescent probe method for the selective determination of adenosine triphosphate(ATP)was developed based on phosphorescent sensitization effect of L-cysteine-triethanolamine capped Mn-doped ZnS quantum dots(Mn:ZnS-TEA QDs).The analytical results show that a linear relationship between the phosphorescence enhanced(?PL)and the concentration of DA is good in concentration range of 0.1?1.0 ?mol/L and in neutral solution.The calibration equation is ?PL=12.50 + 146.42c(ATP)with a correlation coefficient(r)0.993 and a linear range of 0.08?3.0 ?mol/L as well as a detection limit of 0.04?mol/L.The method has been used to determine of ATP in coenzyme tablets with 4.17%RSD and with an average spiked recovery at 93.75%.