Synthesis,Crystal Structure And Antioxidative Activities Of Transition Metal Complexes With Asymmetrical Chiral Schiff Base Ligand

Reactive oxygen species(ROS)mainly includes the hydroxyl free radical,superoxide free radicals and hydrogen peroxide.In the process of cell or tissue of normal physiological metabolism will produceROS,Superoxide dismutase(SOD)is a kind of acidic metal enzyme,widely exists in animals and plants and microorganisms.It can catalytic super oxygen free radicals produce disproportionation reaction into oxygen and hydrogen peroxide,have the effect of anti-aging,improve immunity.However,there are some drawbacks,such as high cost,containing heat factor,poor permeability,short cycle and defects and so on.So its application was restricted.SOD of simulation research attention gradually.Therefore,we have synthesized some molecules with two kinds of schiff base ligands.Their transition metal complexes were synthesized.The structure of these compounds have been determined by X-ray crystallographic methods and characterized by FT-IR,element analyses.These complexes SOD-like activity were tested by xanthine oxidase/nitrotetraolium blue chloride reduction method.Using ABTS method for determining the oxidation resistance of the complexes.Studying the asymmetric catalytic activity of thioether under certain conditions.We also investigated the binding of complexes to BSA by multi-technique spectroscopy.This is benefical for antioxidant and catalytic activity design.In this paper,we have synthesized several transition compounds with double oxalyl dihydrazone and asymmetrical chiral schiff base ligand,in addition,their structure determined by X-ray diffraction analysis.Studies on theses compounds as antioxidant show that,apart from compound land its hybrid proteins show SOD activity.The metal ion of compounds and natural SOD are consistent,manganese complex show higher SOD activity.The ABTS·+radical scavenging capacity showed that,the activity of hybrid proteins and BSA adduction is more obvious than the complexes.In this paper,we also studied the catalytic activity of these complexes and their hybrid proteins.Using the orthogonal experimental design to study the catalytic conditions of methyl phenyl sulfide,separation product by HPLC,the result show that the BSA adduction is more obvious than the complexes.Studing on the interaction between these complexes and BSA,we obtained the quenching constants KSV,binding constants K,binding modes.The secondary structure and the amino acid residues microenvironment of BSA have change in the presence of these complexes.The binding compounds to BSA were static quenching.In our study,we focused on antioxidant and catalytic activity and the interaction with BSA of transition metal complexes.We summarized the correlation of the structure of these complexes with the biological activity.This will be beneficial for design simple and high activity antioxidant and catalyst.