The Preparation And Character- Ization Of Dihydromyricetion Inclusion Complexes

Dihydromyricetin in vine tea has many good pharmacological activity such as antihyperglycemic,antialcoholism,antimicrobial and antioxidant.Because of the poor water solubility,dihydromyricetin cannot be wildly applied in medical field.In order to improve water solubility and bioavailability,dihydromyricetin could be prepared into appropriate dosage forms.In this study,dihydromyricetin was prepared to inclusion complexes with hydroxypropyl-β-cyclodextrin in saturated solution,single-factor analysis were optimized by encapsularion efficiency and water-solubility,the results were as follows:20.0 mL dihydromyricetin was dropping into mixture every minute at 50.0℃,removing solvents at 80.0℃.As a result,the encapsularion efficiency of inclusion complexes was 82.8±1.3%and the luminousness of dihydromyricetin inclusion complexes was 98.0±0.9%.Inclusion complexes were more stable than dihydromyricetin at a high temperature of 45.0±0.2℃.This research also explored mechanism of inclusion complexes,the results showed that alkaline condition and high ionic strength decreased the effect of inclusion.The aqueous solution of inclusion complexes were more steadily in solution which were low ionic strength,nearly neutral and alkaline condition.Solubility curve showed that dihydromyricetin and hydroxypropyl-β-cyclodextrin was combined by as 1:1.Release degree was studyed to indicate release condition in vitro,the results showed that inclusion complexes released rapidly.Rat intestine perfusion was studyed to indicate the intestinal absorption,the results showed that inclusion complexes was easier absorption dihydromyricetin.The absorption equation was y=-0.013x+2.003,R~2=0.904.Bioavailability of dihydromyricetin was increase greatly.