Extraction,purification And Structural Preliminary Identification Of Panax Polysaccharides

In this paper,Panax polysaccharides,including polysaccharides in Panax ginseng C.A.Meyer,Panax quinquefolius L.and Panax notoginseng were separated and purified,and the primary structure was studied.The optimal conditions of sevag was:sevag was added equivalent 1/4 volume of sugar,the volume ratio of chloroform and n-butanol was 5:1,repeated five times,and oscillation 25 min each time.The polysaccharides was deproteinized by method of sevag.Using sephadex G-100,five components were isolated and purified from polysaccharides in Panax ginseng C.A.Meyer,named PPG-1?PPG-2?PPG-3?PPG-4?PPG-5,whose relative molecular mass were 7×104?4×104?1.7×104?1×104?2×103;Five components were isolated and purified from polysa-ccharides named PPQ-1?PPQ-2?PPQ-3?PPQ-4?PPQ-5,whose relative molecular mass were 7.9×104?4.3×104?1.5×104?1.1×104?5.8×102,Two components were isolated and purified from polysaccharides in Panax notoginseng,whose relative molecular mass were 7×104?8.7×102.PPG-2?PPQ-1?PPQ-2?PPN-1 were considered a single component with a homogenous molecular Content ascertained by ultraviolet spectroscopy,sephadex G-100 and HPLC.Finding that the monosaccharide composition of these were mainly composed of glucose by analyzing the complete acid hydrolysates of PPG-2?PPQ-1?PPQ-2?PPN-1 by HPLC.Analyzing the results of the products from malaprade reaction and GC chromatogram of smith degradation products and IR,PPG-2 and PPQ-1 were mainly composed of ?-D-(1?4)or ?-D-(1?6)pyran glucose,both PPQ-2 and PPN-1 were glucan and mainly composed of?-D-(1?4)pyran glucose.