The Accumulation Of Diatery Advanced Glycation End Products And The Damage To Heart In Healthy Rat

Advanced glycation end products(AGEs)are formed by reactions between the carbonyl groups of reducing sugars and the free amino groups on proteins during food heat processing and storage.Dietary intake provide a significant amount of AGEs in body.The AGEs daily intake has been estimated to 25-75 mg,and CML intake was range from 34 to 252 ?g/kgBW.Enhanced AGEs accumulation is associated with the development and pathogenicity of diabetes,diabeticnephropathy and cardiovascular disease.In order to investigate the influence of diatery AGEs on the metabolism of glucose and lipid,two groups of SD rats were fed the high AGEs(H-AGEs)diet and normal AGEs(N-AGEs)diet for 6,12 and 18 w.Carboxymethyllysine(CML),one of representative AGEs was analysed in heart,liver,spleen,lung and kidney to explore the effect of dietary AGEs on the CML accumulation in vivo.The damage of dietary AGEs to heart was also assessed.The main conclusions were as follows.(1)Dietary AGEs can significantly increased the insulin resistance index(HOMA-IR)anddecreased the insulin sensitivity index(ISI)(P<0.05),which improve the insulin resistance.But there is no influence to the glucose tolerance and lip metabolism.(2)Dietary AGEs can significantly increased CML concentration in heart,kindey and liver(P<0.05).CML concentration wassignificantly increased in kindey after 12w's high AGEs diet(P<0.05).CML concentration in heart and liver was significantly increased at18w(P<0.05).There was no obverious change in spleen and lung.CML concentration in vivo from high to low were heart > kindey > liver > spleen > lung.(3)Healthy rats were fed high AGEs diet for 18 w,T-SODand CAT activity were significant decreased(P<0.05),the MDAcontent was significant increased(P<0.05),the dietary AGEs could induce oxidative damage in heart.The inflammation maker:TNF-?was also increased at 18 w(P<0.05),the dietary AGEs also could induce inflammation damage in heart.RAGE concentrationwas also significant increased.