Optimization Of Cellulase Production Conditions Of Neurospora

The filamentous fungus Neurospora crassa is a natural cellulose degrader,which contains a large number of cellulase and hemicellulase genes and grows relatively fast on plant biomass or cellulose.The aim of this study is to optimize the fermentation conditions for Neurospora crassa to produce cellulases.???Effects of individual fermentation conditions,such as inoculation amounts of spores,loading volumes and light,on the cellulase production.?1?When spore concentration reached OD₆₀₀=0.04,cellulase activities of the fermentation media on filter paper and carboxymethyl cellulose?CMC?reached the maximum;?2?when contained 60%of fermentation media,cellulase activities of the fermentation media on filter paper and carboxymethyl cellulose?CMC?reached the maximum;?3?Under the condition of light?300 lux?,dark,or alternating light and dark?12h intervals?,light conditions favored the production of cellulases.???Optimizing fermentation conditions for the production of cellulases by the dual Response Surface Optimization?RSM?technique.Both cellulase activity and protein concentration were used as the response values.Design-Expert was used for experimental design.The derived optimal fermentation conditions were as follows:22.5°C of temperature,6.25 of pH,and 200 rpm of rotation rate.Under the optimal conditions,CMCase reached1.23 g/L,FPase reached 1.55 g/L,and protein concentration reached 162 mg/L.???The third part was the effect of adding surfactants into the fermentation media on cellulase production by wild type Neurospora crassa and its mutant DJS1 with short hyphae.When Triton X-100 was applied,enzyme production did not change much.However,when0.05 or 0.1 g/L of Triton X-100 was added to the fermentation media,cellulase production was significantly increased by over two-fold from the short hyphae mutant.