Dynamic Assembly Of DNA Origami And Its Application In Core-shell Nanoparticles

DNA origami(DNA origami)is a DNA self-assembly technique that uses staples to bind scaffold strand for forming nanostructures by the principle of complementary base pairing.Since Rothemund used DNA origami to construct a variety of two-dimensional patterns,DNA origami greatly promotes the progress of DNA nanotechnology.DNA origami was extremely suitable for preparing controllable pattern of nanostructure,having many advantages compared with other assembly methods.So there are lots of potential applications in numerous areas.The assembly is the basic research of DNA origami,but current assembly method is relatively single,We proposed the dynamic assembly of DNA origami,and used origami as template to prepare core-shell nanoparticles,which could be conducive to the development of the assembly and applications of DNA origami.The main research contents include:1.We proposed a dynamic assembly method for making origami based on the capillary axial temperature gradient using heat gun and got origami structure with a good shape.The temperature gradient of different conditions was simulated and the conditions suitable for flow assembly were determined.The assembly efficiency of the solution at different flow rates was discussed.And the effect of the thermodynamics of the assembly process and the chain displacement reaction was investigated based on the spatial temperature field.2.Core-shell structure nanoparticles based on DNA origami were fabricated.Rectangular DNA origami was used as template for the synthesis of core-shell structure nanoparticles.And SEM and TEM showed that the core-shell structure particles had a good template shape.The XRD and Raman spectra of the core-shell structure nanoparticles after heat treatment showed that the crystal of TiO2 changed to anatase,then the photocatalytic degraded rhodamine B into colorless,which showed good photocatalytic activity.