Study Of Surface Plasmon-Coupled Emission Based On Silver Nanocubes And Molecular Imprinting Membrane

Surface plasmon-coupled emission(SPCE)is a special nano-optical phenomenon which is caused by the near-field interaction between the surface plasmons in metallic nano-film and excited fluorophores.SPCE has the dual advantages of surface plasmon resonance and fluorescence emission.Its spectral response is mainly concentrated at the interface(20-200 nm),which is extremely sensitive to the change of distance between fluorophore and metal surface,combined with metal nanostructures will further improve the fluorescence signal and detection sensitivity.Furthermore,molecular imprinting polymers have attracted the attention of more and more researchers for their good specificity and universality,and have been widely used in medicine,food analysis and environmental monitoring.In recent years,surface molecular imprinting(SMI)has emerged as a new method.This dissertation focused on exploring surface Plasmon coupled emission(SPCE),providing a new idea for sensing analysis.Meanwhile,the molecular imprinting polymers hold the promise for SPCE sensing design.The dissertation consists of four chapters:In the first chapter,a literature summarization is presented.Firstly,the principle,spectral characteristics and research progress of SPCE are introduced.Secondly,an overview of research on thin film coupling "Hot Spot" enhanced fluorescence and its applications are described.Then,the development and application of molecular imprinting technology are summarized.Finally,the plans for the dissertation were put forward.In the second chapter,we studied the film-coupled metal nanocube system with emitters embedded in the spacer layer.Firstly,silver nanocubes with different sizes and uniform shapes are synthesized.We fabricated the film-coupled silver nanocube via the self-assembly process.And "turn off" fluorophores located in the quenching zone of the metallic film were turned on by assembling metallic nanocubes on the film,radiating as SPCE.We investigated the effect of assembly density,coupling distance,dye loading and other factors on SPCE signal.The results showed that when the distance between the nanocubes and the silver film is about 12 nm,the"dequenching" enhancement factor reached?103,which is expected to be used to develop biosensors through macromolecular connection.In the third chapter,surface plasmon-coupled emission based on molecular imprinting membrane was studied.The molecular imprinting polymer was constructed on an SPCE composite substrate.Then the target glycoprotein was specifically identified and recombined with MIPs.The angular resolution and polarization of the signal after capturing the target fluorescent protein were preliminarily investigated.We also used the surface plasmon-coupled emission microscope(SPCEM)to support the results.The results showed the feasibility of combining SPCE and MIPs,which is expected to improve the sensitivity of sensing and built efficient biosensor platform synergistically.In the fourth chapter,we summarized the the main research work and innovation,and the future prospects of the work are described.