Detection Of Residue Psychotropic Drugs And Anesthetics In Foods Of Animal Origin

In animal husbandry industry,psychotropic substances and anesthetics are commonly used in animal growth and transportation.Successive use or abuse of these drugs would lead to physical and psychological dependence.As a result of the abuse,the accumulation of drug residues in animals could be carried through the food chain into the human body,then may endanger human health.Therefore,it is of great significance to establish a rapid,sensitive and accurate detection method to monitor the safety of psychotropic drugs and anesthetics in animal food.This paper focused on developing the method of simultaneous determination of psychoactive drugs in pork by UPLC-ESI-MS/MS and developing the method of simultaneous determination of anesthetics in aquatic products by liquid chromatography-mass spectrometry.The main contents and results of the researches are as follows:(1)Through the optimization of operation parameter and pretreatment method,a method was developed that allows for simultaneous determination of 20 psychoactive drugs residues in pork by solid phase extraction coupled with ultra-performance liquid chromatography with electrospray ionization tandem mass spectrometry.The calibration curves showed a good linearity in the range of 5-100 ng/mL with the correlation coefficients more than 0.990 for the 20 psychotropic drugs.The limits of quantification(LOQ)were 5 ?g/kg for the 20 psychotropic drugs.The average recoveries of the 20 psychotropic drugs in blank pork at 5,10 and 50 ?g/kg spiked levels were 66.8-97.2%,with relative standard deviations from 4.2%to 12.4%.(2)Through the optimization of the operation parameter and condition of chromatography and pretreament method,a simplified method was presented for the simultaneous determination of 3 anesthetics in aquatic products by solid phase extraction coupled with liquid chromatography tandem mass sperctometry.The calibration curves showed a good linearity in the range of 1-1000 ?g/L with the correlation coefficients more than 0.997 for the 3 anesthetics.The limits of quantification(LOQ)were 5 ?g/kg for the 3 anesthetics.The average recovery of 3 anesthetics in blank aquatic products at 5,10,50 and 1000 ?g/kg spiked levels was in the range 63.57-94.55%,with relative standard deviations from 2.62%to 11.35%.