Preparation And Efficacy Evaluation Of Anthocyanin Liposomes

Anthocyanins are widely distributed in plants in the water soluble natural pigment,poor stability,easily degraded or oxidized by temperature,pH,light,metal ions and the influence of external factors,and lipid solubility,anthocyanins utilization rate is not optimistic in vivo,how to increase the stability and improve bioavailability become a hot research of anthocyanins in recent years.The study found that liposome as a double molecule to form stable phospholipid as wall material layer structure can reduce the toxicity of drugs,can have good biocompatibility and cell phospholipid bilayer membrane,and gastrointestinal mucosal cell fusion,endocytosis and phagocytosis,and improve the stability and the absorption rate in core material.In this study,We prepared the anthocyanin liposome with anthocyanin from grape peel,and the entrapment efficiency as indexes,the optimum process for preparing anthocyanin liposome,and optimize the response surface test,determine the best condition of preparation process.Analysis of components of grape anthocyanins,stability of anthocyanins and liposome in different conditions,ROS free radical scavenging activity in vitro cytotoxicity evaluation of anthocyanins and liposome,and comparison of anthocyanins and anthocyanin liposome suspension liquid in gastric parietal cells in the model of transport and absorption efficiency,the main the results are as follows:1.The grape anthocyanins purified as raw material,soybean lecithin and cholesterol as membrane materials,liposome encapsulation rate as the index,based on single factor experiment,response surface optimization test.The optimum process conditions for preparation of grape anthocyanin liposome by film ultrasonic method:The quality of soybean lecithin and cholesterol ratio was 4.2,ultrasonic power was 120 W,the ultrasonic time was 3.12 min,the largest grape anthocyanin liposome encapsulation rate was 40.1%.Under these conditions,the liposomes were large single chamber liposomes with a particle size of 117 nm and a Zeta potential of 8.56 m V.2.In this paper,the stability of anthocyanin and its liposomes in different pH conditions were studied.The rate of decay of anthocyanins in the liposome suspension was lower than that of free anthocyanin in neutral and alkaline conditions.Free anthocyanin degradation by liposome encapsulated will slow rate of anthocyanins in high temperature,light,dark and 4? in the environment,and under this condition,adding VC VE can improve the stability of liposome.3.We get the mainly composed of peonidin-3-5-di-O-glucoside,malvidin-3-5-di-Oglucoside,peonidin 3-O-glucoside and malvidin-3-O-glucoside of the grape anthocyanin by HPLC-DAD-ESI-MS2.Cytotoxicity was detected by SRB on Hep G-2 and MKN-28 cells,the IC50 values were 0.218mg/m L and 0.309mg/m L,experiments show that anthocyanins of the liposome suspension will significantly reduce the cell toxicity.The results of ROS showed that there was no significant difference in antioxidant activity of anthocyanin and anthocyanin liposome suspension.MKN-28 cells were used to simulate gastric absorption,The results showed that the absorption rate of anthocyanins of the liposome suspension was higher than that of free anthocyanins in the same concentration.