Construction Of MOFs Derived Carbon Based Electrochemical Sensors And Research Of Antioxidants

Superoxide anion(O₂^·-)plays an important role in regulating metabolism and physiological function.The accumulation of excessive O₂^·-may lead to some diseases,such as inflammation and cardiovascular disease.Therefore,the construction of O₂^·-sensor is critical.In this paper,the O₂^·-sensor was constructed by MOFs-derived nanoparticle material loaded silver nanoparticles?AgNPs?.And then the scavenging capacity to O₂^·-of food antioxidants was discussed.Finally,the synergistic effect of food antioxidants and SOD enzymes was studied.The details are as follows:?1?A novel non-enzymatic O₂^·-sensor was prepared by AgNPs decorated MOFs compound-derived metal oxide coated carbon material.Transmission electron microscopy?TEM?was employed to characterize morphologies and structures of the as-prepared samples AgNPs/Co₃O₄@C.Electrochemical studies indicated that the AgNPs/Co₃O₄@C/GCE exhibited excellent detection ability to O₂^·-.It offered a linear range of 8 orders of magnitude and a super low detection limits of 0.0564 pM.The sensor was successfully adapted to detection of O₂^·-released from living cells.Furthermore,the capacities of scavenging O₂^·-of food antioxidants were also studied by electrochemical method at cellular milieu.Accordingly,this work provided tremendous potential for reduction incidence of oxidative stress by intaking food antioxidants.?2?It can be concluded from the previous chapter that AgNPs is a good non-enzymatic catalyst,and MOFs derived carbon material is an effective matrix for dispersing AgNPs.However,the preparation process of the experiment in the previous chapter is complex and time-consuming,so we selected the silver nitrate as the source of the metal center and 2-aminoterephthalate as the organic ligand to prepare the Ag-based MOFs.Subsequently,the AgNPs-MC was obtained by calcination at 550?to construct the O₂^·-sensor.AgNPs-MC shows good stability,selectivity,reproducibility and super low detection limits of 0.583 pM toward O₂^·-.Furthermore,the synergistic effect of superoxide dismutase?SOD?and tea polyphenols?TP?to scavenge O₂^·-released from living cells were also studied by electrochemical method.Thus,it is necessary to use MOFs-derived carbon material as a sensing platform to seek further development of antioxidants in oxidative stress related diseases.