Research The Regulation Of Lipid Metabolism Of Anthocyanins From Aronia Melanocarpa

Aronia melanocarpa is a round purple-black small berry.It is rich in various nutrients and biological active ingredients such as phenolic acid,proanthocyanidins,anthocyanins and flavonoids,which have antioxidant,anti-cancer and anti-inflammatory effects.As a kind of natural edible pigment,anthocyanins are widely found in nature,with bright color and strong antioxidant activity.They have many physiological functions and health care value beneficial to human body.In this study,the anthocyanins of Aronia melanocarpa were extracted and purified,establish a high-fat model of mice by high-fat diet,and the effects of anthocyanin from Aronia Melanocarpa on lipid metabolism in high-fat diet mice were studied from physiological and biochemical indexes,molecular mechanism and intestinal microbial structure.(1)It was extracted with 50%ethanol and purified by AB-8 macroporous resin.After extraction with ethyl acetate and n-butanol,the content of anthocyanin from Aronia Melanocarpa(AAM)was the highest,which was 150.96 mg/g;After The extract was administered daily to high-fat diet mice for 12 weeks,compared with the high-fat group,AAM can slow the growth of weight and adipose tissue in mice with high?fat diet,reduce the levels of TC,TG and LDL-C in serum,and increase the content of HDL-C,reduce the content of ALT and AST at the same time.(2)After AAM intervention,it can significantly reduce the content of TC and TG in liver tissue of nice with high-fat diet,reduce the degree of hepatic steatosis,and down-regulate the expression of ACC,C/EBPa and SREBP-1C mRNA in liver tissue,significantly increased AMPKa and ACC protein phosphorylation levels,activated AMPK metabolic pathway to regulate body lipid metabolism levels.At the same time,AAM up-regulated PGCl-?,PPARa mRNA expressions,promoted energy metabolism,and maintained lipid metabolism balance in liver tissue.(3)After AAM intervention,it could significantly slow the growth of adipose tissue in mice with high-fat diet,down-regulate the expression of FABP4 and FAS mRNA in epididymal adipose tissue,and significantly increase the phosphorylation level of AMPKa and ACC protein.AAM can activate the AMPK metabolic pathway to regulate body fat accumulation and metabolism.At the same time,AAM up-regulated the expression of PGC1-?,PPARa mRNA,promoted energy metabolism,which were contributing to the balance of lipid metabolism in epididymis.(4)After AAM intervention,it can reduce the relative abundance of intestinal microbial Proteobacteria in high-fat diet mice,inhibit the production of Bilophila and Helicobacter,improve the structure of bacteria involved in lipid metabolism,reduce the nunber of harmful gut microbiota,and regulate intestinal microbial structure with bilance.The above results show that:After intervention in high-fat diet mice,AAM can effectively slow the growth of weight and adipose tissue in mice with high-fat diet,improve serum index changes,regulate mRNA expression in liver and epididymal adipose tissue,increase AMPKa and ACC protein phosphorylation.AAM can regulate intestinal microbial structure,thereby promoting the balance of body fat metabolism and improving lipid metabolism.