| ObjectiveGlucose and cholesterol are often used as markers in clinical diagnosis,According to previous publications,electrochemical techniques can be considered an effective way to detect various molecules due to their high sensitivity,excellent selectivity and fast response.In general,glucose molecule can be detected by an electrochemical method with the help of an immobilized glucose oxidase?GOD?enzyme.In fact,glucose diagnostic test paper have been widely largely used in commercial application.Cholesterol molecules are determined by cholesterol oxidase?ChOx?,cholesterol esterase?ChEt?,and horseradish peroxidase?HRP?.Nevertheless,there are also some shortcomings in the process of developing an electrochemical-enzymatic sensor system,for instance,short period of validity and high cost.In fact,the properties of oxidase?GOD?can be easily influenced by temperature,pH value,humidity and toxic chemicals.The intrinsic nature of enzymes resulted in the crucial problem of the as-fabricated enzymatic sensors which is their poor instability.Due to their wide applications for organisms,it plays an essential role in the detection of pH armaceutical formulations and biological samples.Currently,it is highly urgent to develop a simple,sensitive and accurate manner to analyze active substances since these kinds of analysis is related to the drug quality control,biomedical diagnostic and other industrial applications which has a great impact on public health concern.Therefore,it is necessary to develop non-enzyme sensors for glucose and cholesterol.Carbon nanomaterials have the advantages of good electrical conductivity,high electrocatalytic activity,strong adsorption capacity,good biocompatibility and so on.Fullerene-based nanomaterials,a member of the carbon material family,have attracted much attention since their discovery.In particular,fullerene C60,a new type of pure carbon molecule.In this paper,C60 nanorods were prepared by special ultrasonic method.The morphologies and properties of the C60 nanorods were investigated by SEM,TEM,XRD and UV-vis spectra.They were applied to the study of electrochemical properties of glucose and cholesterol,providing possible technical and material support for the study of non-enzymatic sensors of the two substances.MethodsIn this work,single-crystalline C60 nanorods were synthesized by ultrasonic method in the C60-saturated m-xylene solution.Materials characterizations of as-preapred C60 nanorods were conducted by X-ray diffraction,scanning electron microscopy,transmission electron microscopy,selected area electron diffraction.The electrochemical properties of as-synthesized C60 nanorods,which was deposited on the glassy carbon electrode,was investigated by cyclic voltammetry?CV?,amperometric?i-t?and differential pulse voltammetry?DPV?method,respectively.Results?1?C60 nanorods have been prepared with a uniform morphology and size.More importantly,as-synthesized sample can be redispersed without aggregation.In fact,the image of C60 nanorods reveals its typical 1D rod shaped structures with an average width about 100-200 nm.?2?C60 nanorods modified glassy carbon electrode showed good electrochemical activity to glucose solution,preliminary research suggests that the concentration from 1.594×10-9mol/l to 9.091×10-5mol/l,showing a linear correlation with high stability,not affected by pH.?3?The C600 nanorosd/phenanthrene/Nafion membrane modified glassy carbon electrode can be used to study the electrochemical properties of cholesterol solution.At pH4.0,the concentration of cholesterol from 7.79×10-6mol/l to 2×10-3mol/l,showing a linear correlation with good stability.ConclusionsIn this work,single-crystalline C60 nanorods were synthesized successfully by ultrasonic method.The morphologies and properties of the C60 nanorods were investigated.The surface of glassy carbon electrode was modified with nanorods and the electrochemical properties of glucose solution were studied.The modified electrode showed good electrochemical activity,high sensitivity,stability and reproducibility in a certain concentration range.It provides a possible application prospect for the preparation of glucose non-enzyme sensor.The glassy carbon electrode modified with C60 nanorods/phenanthroline/Nafion membrane was prepared.The electrode showed good electrochemical activity in cholesterol solution,and the current of the electrode was linearly correlated with the cholesterol content in a certain concentration range.The preparation of C60 nanorods/phenanthroline/Nafion membrane modified glassy carbon electrode provides a new idea for the further preparation of cholesterol non-enzymatic sensors. |
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