Study On Bio-enzymatic Preparation Of Antarctic Krill Protein Powder

This paper mainly uses the degree of hydrolysis as an indicator,Compare the four enzymes to get the best enzymatic enzyme,an the optimal enzymatic conditions were determined by single factor experiments.Next,the Antarctic krill protein is isolated and purified to obtain an Antarctic krill protein peptide.We used Tricine-SDS-PAGE gel electrophoresis to determine the molecular weight of the peptide obtained which Suitable for small peptides.Finally,measured its antioxidant properties.Next,we will make the production and quality analysis of Antarctic krill protein powder.This article is divided into five parts:A: Preparation and process optimization of Antarctic krill protein peptideFirst determine the basic components of the Antarctic krill raw materials,The papain was obtained by screening for hydrolysis degree,and then the single-factor and response surface optimization experiments were carried out to obtain the Antarctic krill enzymatic solution under optimal conditions.B: Defluoridation conditions determination of Antarctic krill enzymatic hydrolysateThe defluorination conditions of the Antarctic krill enzymatic hydrolysate were determined as follows: defluorination with calcium oxide,pH 9.0,water bath temperature35℃,dehydration time in water bath for 1 h.C: Separation,purification and molecular weight analysis of Antarctic krill protein peptideSeparate the Antarctic krill enzymatic hydrolysate which was obtained by enzymatic hydrolysis,three components can be obtained.The three components were separated on a Sephadex G-50 gel filtration column to obtain four separated peaks.The molecular weight of the isolated peak S3 was determined by Tricine-SDS-PAGE gel electrophoresis.Four: biological activity of Antarctic krill protein peptideThe Antarctic krill enzymatic hydrolysate was subjected to the bioactivity assay after the ultrafiltration of the three components and the peak after Sephadex G-50 gel filtration chromatography to filter out the most active antioxidant Antarctic krill peptide.Five: the production of Antarctic krill protein powderThe preparation and quality analysis of protein powder for Antarctic krill enzymatic hydrolysate.Summary: The optimal enzymatic hydrolysis parameter for papain is 3000 U/g protein per 100 g of Antarctic krill meat,the enzymatic hydrolysis temperature is 55℃,the enzymatic hydrolysis pH is 6.0,the enzymatic hydrolysis time is 2 h,and the ratio of material to liquid is 1 : 2.The biological activity of each component was determined by ultrafiltration separation and Sephadex G-50 gel filtration column separation.Preparation of Antarctic krill protein powder using the most biologically active components,spray drying conditions: feed amount 25 %,inlet air temperature 185 ℃,feed temperature30 ℃,feed rate 12 mL / min;Antarctic krill protein powder It is white powdery and has a strong hygroscopicity.